中南民族大学学报（自然科学版）Issue(1)：56-61,6.
大黄素体外抗柯萨奇病毒 B4的实验研究
Antiviral Effect of Emodin on Coxsakievirus B4 in vitro
摘要
Abstract
To investigate the antiviral effect of emodin on Coxsakievirus B4 , ribavirin was used as the positive control and MTT assay was used to evaluate its anti-CVB4 effect through 3 manners including propagation inhibition, direct inactivation and anti-absorption.Then real-time PCR were performed to determine the effect of emodin on the mRNA expression of CVB4 and plaque forming assay were applied to detect the effect of emodin on the plaque formed rate after virus infection at different time.The results indicated that emodin could not directly inactivate the viruses or block their absorption to cells, but it may act as a biological synthesis inhibitor.The IC50 was detected as (14.10 ±0.74) μM and TI were detected as (4.35 ±0.6).Emodin could significantly reduce the mRNA expression of CVB4 gene and may act its inhibitory effect during 0~4 h postinfection(p<0.05).So emodin may act its antiviral activity of inhibiting CVB4 replication after virus penetration in a concentration-and time-dependent manner.关键词
大黄素/柯萨奇病毒B4/抗病毒作用Key words
emodin/Coxsakievirus B4/antiviral effect分类
医药卫生引用本文复制引用
刘钊,马年,钟研,杨占秋..大黄素体外抗柯萨奇病毒 B4的实验研究[J].中南民族大学学报（自然科学版）,2015,(1):56-61,6.基金项目
国家重大新药创制资助项目（2009ZX09301－014－1）；国家自然科学基金资助项目 （）
