中南民族大学学报（自然科学版）Issue(2)：38-43,6.
复方肝舒丸的质量标准研究
Quality standard for Ganshu Pills
摘要
Abstract
Objective:To establish a quality standard of Ganshu Pills.Methods: Radix et Rhizoma Salviae Miltiorrhizae, Radix et Rhizoma Rhei, Radix Angelicae Slinensis , Rhizoma Atractylodis Macrocephalae in Pills are distinguished by TLC.The content of tanshinone IIA was determined by HPLC.The analysis was performed on a Welchrom-C18(5μm,250 mm ×4.6 mm) column with the mobile phase of methanol-water(volume ratio 80∶20), at the flow rate of 1.0 mL· min-1, the Column temperature was 30℃,and the detection wavelength was 270 nm.The content of chrysophanol and emodin were determined by HPLC.The analysis was performed on a Welchrom-C18 (5 μm,250 mm ×4.6 mm ) column with the mobile phase of methanol-0.1%phosphoric acid solution (volume ratio 90∶10), at the flow rate of 1.0mL· min-1, the Column temperature was 25℃, and detection wavelength was 254 nm.Results: The TLC spots were clear, without interference of negative control strong specificity.The linear range of tanshinone IIA was 0.02~0.2μg (r=0.9999), the average recovery rate was 99.26%, RSD was 0.66% (n =9).The linear range of emodin was 0.2 ~2.0 μg(r =0.9999),the average recovery rate was 99.21%, RSD was 0.61%(n=9).The linear range of chrysophanol was 0.2~2.0 μg(r=0.9999),the average recovery rate was 99.21%, RSD was 0.72% (n=9).Conclusion: This method has advantages of simple operation, high sensitivity, accurate results, good reproducibility, specificity can be used as the quality control standard of Ganshu Pills.关键词
复方肝舒丸/TLC/HPLC/质量标准Key words
Ganshu Pills/TLC/HPLC/quality standard分类
医药卫生引用本文复制引用
刘义梅,李孟芝..复方肝舒丸的质量标准研究[J].中南民族大学学报（自然科学版）,2015,(2):38-43,6.基金项目
中国中医科学院基金资助项目 （）
