中山大学学报（自然科学版）Issue(2)：92-100,9.DOI:10.13471/j.cnki.acta.snus.2015.02.019
雷公藤制剂U PLC指纹图谱研究及4种效应成分的含量测定
Fingerprints and Content Determinations of Four Active Components of Tripterygium Preparations by UPLC
摘要
Abstract
To establish fingerprints of Tripterygium preparations from different manufacturers, and to simultaneously determine the contents of four active components by ultra performance liquid chromatogra-phy (UPLC),providing a rapid detection method for quality control of Tripterygium preparations.UPLC separation was performed on a BEH Shield RP 18 column (2.1 mm ×100 mm,1.7 μm)with a mobile phase of acetonitrile-water under gradient elution,the flow rate was 0.25 mL/min and the column tem-perature was 35 ℃,the detection wavelength was set at 220 nm.The similarity of UPLC fingerprints was evaluated by qualitative and quantitative analysis,respectively.The results showed that the fingerprints of Tripterygium preparations from different manufacturers were found to be in great diverse,while the contents of four active components were significantly different.So,this method is specific,fast,and with good resolution for the determination of UPLC fingerprint of Tripterygium preparations.The simulta-neous determination of contents of four active components can be used for the quality control of Tripterygi-um preparations to ensure the safety and utility in clinical medication.关键词
雷公藤制剂/雷公藤内酯醇/指纹图谱/超高效液相色谱法 (UPLC)Key words
Tripterygium preparation/triptolide/fingerprint/UPLC分类
药学引用本文复制引用
邓金宝,徐方方,徐鹏,毛炜,邓远辉,刘博..雷公藤制剂U PLC指纹图谱研究及4种效应成分的含量测定[J].中山大学学报（自然科学版）,2015,(2):92-100,9.基金项目
国家自然科学基金青年科学基金资助项目（81202398）；国家中医药管理局基地科研专项资助项目（JDZX2012010）；中科院上海药物研究所新药研究国家重点实验室开放研究课题资助项目（SIMM1106KF－12）；广州市科技攻关资助项目 （）
