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利用ISSR分子标记研究樱桃李野生类型的遗传多样性及亲缘关系

李玲 黄峥 崔大方 王永刚 许正 贺涛

中山大学学报(自然科学版)Issue(2):112-117,6.
中山大学学报(自然科学版)Issue(2):112-117,6.DOI:10.13471/j.cnki.acta.snus.2015.02.022

利用ISSR分子标记研究樱桃李野生类型的遗传多样性及亲缘关系

Genetic Diversity and the Genetic Relationship Among Wild Types of Prunus divaricata By Using ISSR Molecular Markers

李玲 1黄峥 1崔大方 1王永刚 2许正 3贺涛1

作者信息

  • 1. 华南农业大学林学院,广东 广州510642
  • 2. 新疆农业科学院,新疆 乌鲁木齐830091
  • 3. 新疆伊犁哈萨克自治州林业科学研究院,新疆 伊宁835000
  • 折叠

摘要

Abstract

This paper used ISSR Molecular Markers to study the genetic diversity and the genetic rela-tionship among 40 types of Prunus divaricata which originated in Tianshan Mountain of China.The study of genetic diversity and the analysis of UPGMA cluster showed that the 40 types of P.divaricate have ge-netic diversity,the average diversity index (I)was 0.488,the genetic similarity coefficient (Hs)ranged from 0.504 to 0.85 1 with an average of 0.678,the genetic distance (GD)varied between 0.127 0 and 0.604 2,the number of alleles (Na)and effective alleles (Ne)was 1.908 and 1.571 respectively,and the population has a low genetic diversity because of the average Nei's gene diversity index and the aver-age Shannon's information index (I)was 0.17 and 0.22;The 40 types of P.divaricate can be classified into 4 groups from the clustering results of molecular markers,combined with morphological analysis.The results showed that the 40 types of P.divaricate can be divided into four varieties,which were included P.divaricata var.humila (N.R.Cui et L.Wang) D.F.Cui,comb.nov.,P.divaricata var.ni-grooarp (N.R.Cui et L.Wang)D.F.Cui,comb.nov.,P.divaricata var.phoenicea (Z.Xu)D. F.Cui,comb.nov.and P.divaricata var.luteola (N.R.Cui)D.F.Cui,comb.nov..

关键词

樱桃李/分子标记/遗传多样性/亲缘关系

Key words

Prunus divaricata/microsatellite markers/genetic diversity/genetic relationships

分类

农业科技

引用本文复制引用

李玲,黄峥,崔大方,王永刚,许正,贺涛..利用ISSR分子标记研究樱桃李野生类型的遗传多样性及亲缘关系[J].中山大学学报(自然科学版),2015,(2):112-117,6.

基金项目

国家自然科学基金资助项目 ()

中山大学学报(自然科学版)

OA北大核心CSCDCSTPCD

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