中山大学学报(自然科学版)Issue(4):112-120,9.DOI:10.13471/j.cnki.acta.snus.2015.04.021
珠江流域黄颡鱼群体的形态度量学与遗传多样性分析
Morphology and Genetic Diversity Analysis of Pelteobagrus fulvidraco Populations in Zhujiang River
摘要
Abstract
Genetic differences of Pelteobagrus fulvidraco populations from Zhujiang River were compared by morphological and molecular biological methods.The scatter diagram of principal component analysis revealed that the populations from Duliujiang River and Lijiang River pooled into one group,while those from Dongjiang River separated from Duliujiang River and Lijiang River.It indicated that Dongjiang Riv-er population had a certain morphological differentiations compared to other two populations,while there were few morphological differentiation between Duliujiang River and Lijiang River.The mtDNA D-loop sequence analysis of three populations showed that 176 variable sites and 36 haploid types were detected, there were similar base compositions among three populations,the content of A +T (59.6%)was high-er than the content of G +C (40.4%),the average gene flow was 3.234,there was a large gene ex-change between three populations.The NJ tree of haploid type shows that the populations Lijiang River and Duliujiang River formed the first cluster which together with the populations from Dongjiang River formed the second cluster.The analyzed result of 21 microsatellite loci in three populations showed that 15 loci were high polymorphic (PIC >0.5),the clustering result of three populations was in accordance with the result of mtDNA D-loop marker,all clustering results had a certain correlation with their geo-graphical distribution.From the result of morphological and molecular analysis,a conclusion can be drawn that the morphology in three populations had certain difference,there was rich genetic diversity a-mong populations.关键词
黄颡鱼/珠江流域/形态度量学/mtDNA D-loop/微卫星Key words
Pelteobagrus fulvidraco/Zhujiang River Area morphology/mtDNA D-loop/microsatellites分类
农业科技引用本文复制引用
谢堂晖,赵会宏,杨慧荣,刘丽,韩兴鹏,杨宪宽,陈奕彬,刘丽杰..珠江流域黄颡鱼群体的形态度量学与遗传多样性分析[J].中山大学学报(自然科学版),2015,(4):112-120,9.基金项目
公益性行业(农业)科研专项经费资助项目(201203066);广东省教育部产学研结合资助项目(2012B091100484);广东省教育部科技部产学研结合资助项目(2012B090600052);广东省科技计划资助项目(2012B040400004);广东省海洋渔业科技推广专项资助项目(A201201E04);广东省协同创新与平台环境建设资助项目 ()