作物研究Issue(3)：231-235,5.DOI:10.3969/j.issn.1001-5280.2014.03.01
水稻Pi9基因序列标记的开发及其抗瘟育种应用
Development of the Pi9 Gene Marker and Its Breeding Practice in Rice Blast Resistance
摘要
Abstract
To improve blast resistance of seven receptor indica rice lines (316B,Jin23B,R207,R228,R288,R389 and Minghui86),a DNA marker,pi9utr was developed based on the 3′UTR sequence of the broad-spectrum blast resistance gene Pi9 and marker-assisted selection breeding practice was implemented in this study.The main results were as fol-lows:Pi9utr was a stable polymorphic dominant marker for six of seven tested crosses except R207 ×75 -1 -127.The se-lection efficiency of the pi9utr marker for blast resistance phenotype was 100%,and the Pi9 gene showed high level expres-sion in all resistant plants,according to randomly sampled individuals analyses of two backcross populations,316B ×75 -1-127 BC4F1 and R288 ×75 -1 -127 BC6F1.Pi9utr was employed for marker-assisted selection in consecutive back-cross breeding of the six receptor rice lines,and BC4 F1 populations of three combinations and BC6 F2 populations of others three combinations were obtained,which provided basis for breeding of new rice varieties with blast resistant.关键词
水稻/稻瘟病抗性/Pi9基因/分子标记辅助选择育种Key words
Rice/Blast resistance/Pi9 gene/Marker-assisted selection breeding分类
农业科技引用本文复制引用
杨婷婷,刘建丰,王国梁,刘雄伦,谭令辞,行璇,文婷,梁毅,吴俊,刘金灵,戴良英..水稻Pi9基因序列标记的开发及其抗瘟育种应用[J].作物研究,2014,(3):231-235,5.基金项目
国家自然科学基金（31171526）；国家转基因生物新品种培育重大专项（2013ZX08001-002）；教育部高校科技创新团队项目。 （）
