作物研究Issue(3)：242-245,4.DOI:10.3969/j.issn.1001-5280.2014.03.03
种子芥酸和硫苷含量对甘蓝型油菜遗传转化的影响
Effects of Erucic Acid and Glucosinolate Content in Seeds on Genetic Transformation in Brassica napus L.
摘要
Abstract
In this study,the lower glucosinolate and lower erucic acid content varieties of Brassica napus L. Xiangyou 15 and Westar,higher glucosinolate and higher erucic acid content strains of Brassica napus GX-272 and GX29 were selected as the experimental materials. The cotyledons petioles from these four cultivars were transformed with bar gene from vector of pFGC5941 by agrobacterium-mediated method. According to the number of PPT-resistent plantlets and PCR tests, cotyledon petiole differentiation rate and transformation rate of four materials were counted respectively. The results showed that cotyledon petiole differentiation rate of Xiangyou 15 and Westar was higher than that of GX-272 and GX29,while the transformation rate was contrary. In this study,glucosinolate and erucic acid content in rapeseed seed affected on cotyledon petiole differentiation rate and transformation rate was preliminarily confirmed.关键词
甘蓝型油菜/硫苷/芥酸/遗传转化Key words
Brassica napus L./Glucosinolate/Erucic acid/Genetic transformation分类
农业科技引用本文复制引用
魏解冰,肖文娟,刘春林..种子芥酸和硫苷含量对甘蓝型油菜遗传转化的影响[J].作物研究,2014,(3):242-245,4.基金项目
国家自然科学基金项目（31071455）。 （）
