作物学报Issue(8)：1356-1363,8.DOI:10.3724/SP.J.1006.2014.01356
TRV病毒介导的基因沉默体系在棉花中的建立及应用
Establishment and Application of TRV-Mediated Virus-Induced Gene Silencing in Cotton
摘要
Abstract
Using upland cotton GhCLA1 as marker gene, tobacco rattle virus induced genes silencing (TRV-VIGS) was estab-lished in cotton. A gene fragment of TRV subgenomic RNA2 was amplified by reverse transcription-polymerase chain reaction (RT-PCR) in root, stem and leaf of cotton, demonstrating the virus can spread into various organs. The TRV induced silencing of CLA1 gene was further tested in 34 different cotton varieties (lines) originated from several ecological regions of China. The re-sults showed that CLA1 could be silenced in all tested varieties (lines), though the levels of silencing showed a little difference, implicating wide application perspective of TRV-VIGS system in cotton. GhMAPKKK, a mitogen-activated protein kinase (MAPK) kinase kinase gene of cotton, was up-regulated in cotton at 96 hours post inoculation by Verticillium dahliae. Silencing GhMAPKKK in G. barbadense cv. Hai 7124, a cotton variety with high resistance to V. dahlia exhibited reduced resistance to V. dahliae infection, suggesting that GhMAPKKK participated in cotton resistance signaling pathway to V. dahliae. With the wide adaption without genotype selection, sensitivity and high throughput, the TVR-VIGS system will significantly promote functional gene analysis in cotton.关键词
棉花/TRV-VIGS/GhCLA1/GhMAPKKK/黄萎病Key words
Cotton/TRV-VIGS/GhCLA1/GhMAPKKK/Verticillium wilt引用本文复制引用
王心宇,吕坤,蔡彩平,徐君,郭旺珍..TRV病毒介导的基因沉默体系在棉花中的建立及应用[J].作物学报,2014,(8):1356-1363,8.基金项目
本研究由国家自然科学基金项目(31171590)和江苏省自然科学基金项目(BK2010065)资助。 （31171590）
