作物学报Issue(9)：1540-1548,9.DOI:10.3724/SP.J.1006.2014.01540
棉花GhMYB0基因的克隆、表达分析及功能鉴定
Cloning, Expression, and Functional Analysis of GhMYB0 Gene from Cotton (Gossypium hirsumtum L.)
摘要
Abstract
MYB transcription factor, one of the most important protein families in plants, is involved in the regulation of secon-dary metabolism, morphogenesis of plant, responding to environment stress and plant hormone. In this study, we used D5 genomic bank of Gossypium raimondii as the reference to AtMYB0 (GL1, NM_113708) protein, and cloned the full-length cDNA of a new MYB transcription factor gene GhMYB0 from cotton (Gossypium hirsutum L.). The open reading frame of GhMYB0 is 843 bp in length, which encodes 280 amino acid residues. GhMYB0 was confirmed as R2R3-MYB transcription factor via conserved struc-ture analysis and subcellular localization. The qRT-PCR result indicated that GhMYB0 was highly expressed at the blossom day, its expresssion amount reached the peak after 20 days, with the most amount in flower, then in ovules. Transgenic funtion analysis indicated that GhMYB0 over-expressed in Arabidopsis lines, showing fewer trichomes in leaf epiderm than in that of the wild type, which suggests that the gene can restore the notrichome phenotype of gl-1 mutant of Arabidopsis. In addition, the transgenic lines had shorter plant heights, longer vegetative growth time, and pollen abortion.关键词
棉花/MYB转录因子/植物表达载体构建/遗传转化Key words
Cotton/MYB transcription factor/Construction of plant expression vector/Genetic transformation引用本文复制引用
王诺菡,喻树迅,于霁雯,吴嫚,马启峰,李兴丽,裴文锋,李海晶,黄双领,张金发..棉花GhMYB0基因的克隆、表达分析及功能鉴定[J].作物学报,2014,(9):1540-1548,9.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2010CB126006)资助。 （973计划）
