作物学报Issue(10)：1725-1731,1732,8.DOI:10.3724/SP.J.1006.2014.01725
以关联分析发掘小麦整穗发芽抗性基因分子标记
Exploring Molecular Markers of Preharvest Sprouting Resistance Gene Using Wheat Intact Spikes by Association Analysis
摘要
Abstract
To improve pre-harvest sprouting (PHS) resistance in wheat breeding, it is important to explore marker loci and elite alleles associated with PHS resistance using intact spikes. In this study, a total of 181 markers were used to 264 genotype materi-als. General and mixed linear models (GLM and MLM) were used to analyze PHS phenotypic data in three environments (2012-in house, 2013-in house and 2013-in field). The results showed that twenty markers were identified by association analysis, and lo-cated on chromosomes 1AS, 2DS, 3AS, 3BL, 4AL, 5AS, 5BL, 6BS, 6DS, 7AL, and 7BL. The markers gwm102 on 2DS and barc340 on 7BL were detected stably in three environments, among which barc340 was likely to be novel and needs to be further studied through biparental linkage mapping analysis. Six markers were detected in two environments, and the other loci linked with 12 markers were detected only in one environment. A total of ten elite alleles were further explored among the eight loci with repeated associations. The alleles barc28-229bp and barc28-217bp for high PHS resistance were all distributed in local cultivars (e.g. Suiningtuotuomai). However, the alleles gwm102-142bp and barc186-199bp with intermediate PHS resistance were mainly detected in released cultivars (e.g. Yangmai 158), which could be beneficial to wheat molecular breeding.关键词
小麦/穗发芽/休眠/分子标记/关联分析Key words
Triticum aestivum L./Preharvest sprouting/Dormancy/Molecular marker/Association analysis引用本文复制引用
朱玉磊,张海萍,王升星,赵良侠,张德新,胡建帮,曹雪连,杨亚杰,常成,马传喜..以关联分析发掘小麦整穗发芽抗性基因分子标记[J].作物学报,2014,(10):1725-1731,1732,8.基金项目
本研究由国家现代农业产业技术体系建设专项,安徽省小麦产业技术体系建设专项和教育部高等学校博士学科点专项(20113418120004)资助。 （20113418120004）
