作物学报Issue(11)：1905-1913,9.DOI:10.3724/SP.J.1006.2014.01905
利用病毒介导基因沉默方法研究小麦抗光氧化相关基因
Functional Analysis of Photo-Oxidative Stress Responsive Genes in Wheat Using Virus-Induced Gene Silencing System
摘要
Abstract
Functional analysis of photo-oxidative stress responsive genes in wheat (Triticum aestivum L.) may benefit wheat im-provement for high radiation use efficiency. A Chinese variety Xiaoyan 54 developed from distant hybridization between common wheat (T. aestivum, 2n=42) and tall wheatgrass (Thinopyrum ponticum, 2n=70) shows significant tolerance to high light induced photo-oxidative stress. Based on previous transcriptome analysis of Xiaoyan 54 in response to high light stress, six genes were selected in this study to assess their possible roles in photo-oxidative stress response using barley stripe mosaic virus (BSMV) mediated virus-induced gene silencing (VIGS) system in Xiaoyan 54. The BSMV induced silencing of the targeted genes together with the BSMV:GFP control plants were exposed to low temperature and high light (LTHL), N-(3,4-dichlorophenyl)-N’,N’-dimethylurea (DCMU), methylviologen (MV), and hydrogen peroxide (H2O2) stress, respectively. The maximum photochemical efficiency of photosystem II (Fv/Fm), the photosynthetic performance index (P.I.), malondialdehyde (MDA) content, and biomass were evaluated. The results showed that Ta23008 and Ta92165 were involved in the responses of wheat to LTHL, DCMU, MV, and H2O2, respectively. Ta106078 was responsible for wheat tolerance to DCMU, MV, and H2O2 while Ta27787 was responsible for LTHL, DCMU, and H2O2 stress. Ta24695 participated in the response of wheat to both LTHL and H2O2. However, Ta119251 seemed to be only responsible for the DCMU stress in wheat. Additionally, four genes, Ta23008, Ta92165, Ta106078, and Ta119251, were likely to regulate biomass accumulation because the biomass was significantly reduced when they were silenced in wheat. These results provided new hints toward understanding the molecular mechanism of tolerance to photo-oxidative stress in Xiaoyan 54.关键词
小麦/大麦条斑病毒(BSMV)/病毒介导的基因沉默/基因功能/光氧化Key words
Triticum aestivum/Barley stripe mosaic virus/VIGS/Genomic function/Photo-oxidative stress引用本文复制引用
陈坤梅,李宏伟,林凡云,陈耀锋,李滨,郑琪,李振声..利用病毒介导基因沉默方法研究小麦抗光氧化相关基因[J].作物学报,2014,(11):1905-1913,9.基金项目
本研究由国家自然科学基金项目(31371609)资助。 （31371609）
