作物学报Issue(11)：1946-1955,10.DOI:10.3724/SP.J.1006.2014.01946
水稻叶片早衰突变体osled的生理特征与基因定位
Physiological Characteristics and Gene Mapping of a Leaf Early-Senescence Mutant osled in Rice
摘要
Abstract
Leaf early senescence directly affects crop yield and quality, therefore, it is of great importance to study its molecular and physiological mechanisms. In this study, we obtained a leaf early senescence mutant, osled, by irradiating rice variety 93-11 with 60Co-γray. The mutant showed early senescence symptoms, such as grey leaf tip and edge and red-brown lesions on leaf, even at tillering stage. Under the condition of simulated drought stress, seedlings of the mutant appeared in obviously shortened plant height and root length. Besides, physiological and biochemical indices in the mutant varied significantly. Under drought stress, the wild type maintained stable levels of malondialdehyde (MDA) content and activities of superoxide dismutase (SOD) and peroxidase (POD) in the top three leaves; however, the mutant showed significantly increased MDA content and SOD and POD activities in these leaves. Although the contents of soluble protein and chlorophyll and catalase (CAT) activity in the top three leaves decreased in both wild type and the mutant, the reductions in the second-top and third-top leaves of the mutant were significantly greater compared to those of the wild type. The results of histochemical staining by trypan blue, 3,3’-diaminobenzidine (DAB), and nitro blue tetrazolium (NBT) disclosed the destruction of membrane system and the accumula-tion of H2O2 and O2܋in the mutant. Besides, ultrastructure of mesophyll cells further explained the degradation of chloroplast in the mutant. A single recessive gene was found to control the senescence trait according to genetic analysis, and the OsLED gene was mapped in the marker interval RM15528–RM15553 on the long arm of chromosome 3, with bilateral genetic distance of 0.7 cM.关键词
水稻/叶片早衰/模拟干旱胁迫/生理分析/精细定位Key words
Rice/Leaf early senescence/Simulated drought stress/Physiological analysis/Fine mapping引用本文复制引用
赵晨晨,黄福灯,龚盼,杨茜,程方民,潘刚..水稻叶片早衰突变体osled的生理特征与基因定位[J].作物学报,2014,(11):1946-1955,10.基金项目
本研究由国家自然科学基金项目(30700498和31271691)资助。 （30700498和31271691）
