作物学报Issue(12)：2090-2097,8.DOI:10.3724/SP.J.1006.2014.02090
水稻糖苷水解酶基因 OsBE1在叶绿体发育中的功能
Functional Characterization of the Glycoside Hydrolase Encoding Gene OsBE1 during Chloroplast Development in Oryza sativa
摘要
Abstract
Chloroplast plays an important role in plant carbohydrate metabolism; however the function of carbohydrate metabo-lism in the chloroplast development is poorly understood. The Oryza sativa Branching Enzyme 1 (OsBE1) gene, encoding a gly-coside hydrolase family 13 protein, was cloned from rice. The identity between OsBE1 and Arabidopsis AtBE1 is 66%, but only 40% between OsBE1 and the classical starch branching enzymes in rice. A T-DNA insertion mutant of OsBE1 gene was identified. The seedlings of the osbe1 mutant were albino, and died at the three-leaf stage. This albino phenotype could not be rescued by exogenous carbohydrate. Starch staining showed no obvious difference in starch content between osbe1 and the wild type. Further studies showed that there were less chloroplasts in osbe1 mutant than in wild type, and there was no obvious stroma lamella in the osbe1 chloroplast. The overexpression vector pCAMBIA1300-35S-OsBE1 was constructed and transformed into rice variety Zhonghua 11. Finally, 108 transgenic lines were obtained and 77 lines of them showed etiolation in different degrees. The work not only sheds a novel insight into the regulation mechanism of carbohydrate metabolism on chloroplast development, but also lays a foundation for further understanding of the OsBE1’s function.关键词
糖苷水解酶/碳水化合物代谢/OsBE1/叶绿体/水稻Key words
Glycoside hydrolase/Carbohydrate metabolism/OsBE1/Chloroplast/Rice (Oryza sativa L.)引用本文复制引用
王兴春,王敏,季芝娟,陈钊,刘文真,韩渊怀,杨长登..水稻糖苷水解酶基因 OsBE1在叶绿体发育中的功能[J].作物学报,2014,(12):2090-2097,8.基金项目
本研究由山西省自然科学基金项目(2013011028-1),水稻生物学国家重点实验室开放课题(090203)和山西省人才引进与开发专项资金资助。 （2013011028-1）
