作物学报Issue(12)：2118-2127,10.DOI:10.3724/SP.J.1006.2014.02118
古蔺牛皮茶种质资源遗传多态性
Genetic Diversity of Gulin-niupicha [Camellia sinensis (L.) O. Kuntze] Germ-plasm Resources
摘要
Abstract
Gulin-niupicha (Camellia sinensis) is a tea landrace population specifically grown in Jiaozigou, Gulin County, Sichuan Province, China. To protect and utilize Gulin-niupicha resources effectively, we investigated the morphologic characteristics, bio-chemical components, and genetic diversity based on inter-simple sequence repeat (ISSR) markers by sampling 76 individuals from seven populations of Gulin-niupicha germplasm. The results showed that: (1) there were various biological characteristics among the seven populations. Importantly, a majority of Gulin-niupicha were shrubs or semi-arbor. Their mature leaves were me-dium in size, elliptic or oblong in shape, and in green or dark green color; while the young shoots showed more colors varying form whitish, yellow green, green or purple green. (2) In the seven populations, two had high contents of free amino acids (>4.5%) and two had both high contents of tea polyphenols (>27% in spring shoots and >31% in summer shoots) and catechins (>150 mg g–1 in spring shoots and >190 mg g–1 in summer shoots). The ratio of polyphenols-to-free amino acids ranged from 3.84 to 16.95 in the seven populations. The results of biochemical component measurements indicated that the Gulin-niupicha germplasm re-sources are of great value in tea tree breeding and tea processing. (3) A total of 135 fragments were amplified in the 76 accessions using 15 pairs of ISSR primers, in which 90.4% (122/135) were polymorphic bands. The genetic diversity of the Gulin-niupicha germplasm revealed by ISSR markers was low, with the Nei’s gene diversity index of 0.254 and Shannon’s information index of 0.392.关键词
古蔺牛皮茶/种质资源/遗传多态性/ISSRKey words
Gulin-niupicha (Camellia sinensis L.)/Germplasm resources/Genetic diversity/ISSR引用本文复制引用
刘绍杰,迟琳,谢文钢,韩楠,陈应会,何鑫,唐茜..古蔺牛皮茶种质资源遗传多态性[J].作物学报,2014,(12):2118-2127,10.基金项目
本研究由四川省科技支撑计划项目(2014NZ0105)资助。 （2014NZ0105）
