作物学报Issue(12)：2176-2182,7.DOI:10.3724/SP.J.1006.2014.02176
小麦籽粒淀粉分支酶同工酶结构组成及时空表达
Constitution and Spatiotemporal Expression of Starch Branching Enzyme in Developing Wheat Grain
摘要
Abstract
This study aimed at disclosing the enzymatic mechanism in amylopectin synthesis in wheat (Triticum aestivum L.). The isozyme forms, organ localization, spatiotemporal expression profile and subunits constitution of starch branching enzyme (SBE) were identified in eight wheat cultivars from different provenances using native polyacrylamide gel electrophoresis (Native-PAGE) and SDS-PAGE. Four SBE isozymes were detected in wheat endosperm, in which isozymes B and SBEIIa were localized in en-dosperm and leaf, whereas isozymes A and Di were exclusively present in endosperm. In the process of grain filling, Di and SBEIIa expressed first, followed by isozyme B, and isozyme A expressed finally. However, B and SBEIIa terminated to express at late filling stage. All SBE isozymes were composed of one subunit of 86–92 kD, and their spatial localization exhibited organ specificity. According to the expression level, Di, B, and SBEIIa are considered as dominant isozymes for grain endosperm de-velopment. They probably determinate the total SBE activity and serve as key factors in amylpectin biosynthesis in wheat grain and leaf.关键词
小麦/淀粉分支酶/支链淀粉/时空表达/器官分布Key words
Triticum aestivum L./Starch branching enzyme/Amylpectin/Spatiotemporal expression/Organ localization引用本文复制引用
刘正帅,刘贵芬,杨明煜,贾晓,李运祥,赵法茂..小麦籽粒淀粉分支酶同工酶结构组成及时空表达[J].作物学报,2014,(12):2176-2182,7.基金项目
本研究由山东省自然科学基金项目(ZR2010CM034)和2013年全国大学生创新创业训练计划资助。 （ZR2010CM034）
