作物学报Issue(1)：15-21,7.DOI:10.3724/SP.J.1006.2015.00015
中品03-5373对大豆胞囊线虫3号生理小种免疫抗性的遗传解析
Genetic Analysis of Immunity to Soybean Cyst Nematode Race 3 in Elite Line Zhongpin 03-5373
摘要
Abstract
Soybean cyst nematode (SCN) race 3, one of the eight races identified in China, is widely distributed and severely re-duced soybean yield. Zhongpin 03-5373 (ZP03-5373) is an elite line immune to SCN race 3. In this study, a recombinant inbred line (RIL) population was developed from a cross between ZP03-5373 and Zhonghuang 13 (ZH13). A genetic linkage map was constructed using a total of 506 molecular markers, including SSRs, EST-SSRs, InDel, and SNP. The total length of the genetic map was 2651.90 cM with an average marker spacing of 5.24 cM. Based on the phenotyping data, we detected three QTL inter-vals to dominate SCN3, includingSCN3-7 (Gm07),SCN3-11(Gm11), andSCN3-18(Gm11). Main effect QTL,SCN3-18 could explain 29.5% of resistant variation. Two minor effect QTLs,SCN3-7 andSCN3-11, explained 6.2% and 5.5% of resistant varia-tion, respectively. And it further showed there was significant epistatic interaction betweenSCN3-7 and SCN3-18 for resistance to SCN3. BothSCN3-7 andSCN3-11 were confirmed to be resistant to SCN3 by tracking flanking marker in the ancestors of ZP03-5373. These markers will be helpful for developing SCN resistant cultivars and cloning resistant genes by marker assisted selection.关键词
大豆/大豆胞囊线虫/重组自交系/分子标记/QTL定位Key words
Soybean/Soybean cyst nematode/Recombinant inbreed line/Molecular marker/QTL引用本文复制引用
刘波,李英慧,于佰双,王家军,刘玉林,常汝镇,邱丽娟..中品03-5373对大豆胞囊线虫3号生理小种免疫抗性的遗传解析[J].作物学报,2015,(1):15-21,7.基金项目
本研究由国家自然科学基金项目(31171575)和国家科技支撑计划项目(2013BAD01B03)资助。 （31171575）
