作物学报Issue(1)：31-41,11.DOI:10.3724/SP.J.1006.2015.00031
花生转录因子基因NAC4的等位变异分析
Allelic Variation of Transcription Factor GenesNAC4 inArachis Species
摘要
Abstract
NAC transcription factors play an important role in response to abiotic stress of plant. In this paper, bioinformatic analysis indicated that transcription factor geneAhNAC4(accession number HM776131.1) can response to drought signal. The comparison of cDNAs (ShrNAC4-a andShrNAC4-b) and genomic DNAs (ShNAC4-a andShNAC4-b) from Shanhua 11 showed thatAhNAC4 has a full length of 1244 bp and an ORF of 1050 bp, containing two introns at 182–279 bp and 547–642 bp, and encoding 349 amino acids. Four kinds ofAhNAC4 genes, named asAhNAC4-a1, AhNAC4-a2, AhNAC4-b1, andAhNAC4-b2(ab-breviations:a1, a2, b1, andb2), were cloned from 32 cultivars with different drought resistances. Among them a1 anda2 were alleles, with only one locus different between the two genes, leading to the difference of corresponding amino acids at site 174;b1 andb2 were alleles, with 14 SNPs, of which two SNPs led to differences of the corresponding amino acids at sites 174 and 244. There were four genotypes ofAhNAC4 in the 32 cultivars, containing 10a1a1b1b1, 5a1a1b2b2, 15a2a2b1b1, and 2a2a2b2b2. Meanwhile, 11NAC4 genes (Aw1NAC4–Aw11NAC4) were isolated from 19 wild peanut accessions inArachis.Aw2NAC4 had the highest homology of nucleotide sequence with a1 ora2, andb1 orb2 had the highest homology of nucleotide sequence with Aw1NAC4. It is speculated that the protein encodinga1 plays a key role in responding drought stress, and the function of the pro-teins encodinga1 andb1 genes is closer with that of wild species.关键词
花生/转录因子/NAC/等位变异Key words
Peanut/Transcription factor/NAC/Allelic variation引用本文复制引用
李文,万千,刘风珍,张昆,张秀荣,厉广辉,万勇善..花生转录因子基因NAC4的等位变异分析[J].作物学报,2015,(1):31-41,11.基金项目
本研究由山东省花生良种产业化工程项目,山东省现代农业产业技术体系花生创新团队建设项目(SDAIT-05-011-02),国家现代农业产业技术体系建设专项(CARS-14),国家“十二五”科技支撑计划项目(2011BAD35B04)和国家自然科学基金项目(31271757)资助。 （SDAIT-05-011-02）
