作物学报Issue(1)：49-56,8.DOI:10.3724/SP.J.1006.2015.00049
小麦-中间偃麦草渗入系抗白粉病基因PmCH7124的分子定位
Molecular Mapping of Powdery Mildew Resistance GenePmCH7124 in a Puta-tive Wheat-Thinopyrum intermedium Introgression Line
摘要
Abstract
Wheat introgression line CH7124 derived from a cross between wheat–Thinopyrum intermedium line TAI8335 (resis-tant to powdery mildew) and common wheat variety “Jinmai 33” (susceptible to powdery mildew) exhibits immunity toBlumeria graminisf. sp.tritici (Bgt) pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at the seedling stage. TheBgt resistance in CH7124 is similar to that in TAI8335 and its wild parentTh. intermedium. However, noTh. intermedium chromatinhas been detected ac-cording to genome in situ hybridization (GISH) assay. In this study, we determined the single dominantBgt resistance gene in CH7124, tentatively designatedPmCH7124, using populations derived from SY95-71/CH7124 (F6) and CH7124/Mianyang 11 (F1 and F2). Five SSR markers (Xgwm47,Xgwm120,Xwmc332,Xgwm501, andXbarc101) were identified to be codominant with PmCH7124 according to bulked segregant analysis, and the closely flanking markers wereXgwm501 andXbacr101with genetic distances of 1.7 cM and 4.5 cM, respectively. The target resistance gene was chromosomally located on 2BL with Chinese Spring nulli-tetrasomicand ditelosomic lines. We primarily consider thatPmCH7124is a newBgt resistance gene because its resistance spectrum, origin, chromosomal location, and linked markers are different from those of the knownBgt resistance genes, such as Pm6,Pm33,PmJM22,MlZec1,MlAB10, andMlLX99.关键词
小麦-中间偃麦草渗入系/白粉病抗性/GISH/SSR标记/连锁图谱Key words
Wheat-Th. intermediumintrogression line/Resistance to powdery mildew/GISH/SSR markers/Linkage map引用本文复制引用
李建波,乔麟轶,李欣,张晓军,詹海仙,郭慧娟,任永康,畅志坚..小麦-中间偃麦草渗入系抗白粉病基因PmCH7124的分子定位[J].作物学报,2015,(1):49-56,8.基金项目
本研究由国家自然科学基金项目(31171839),山西省国际科技合作计划项目(2013081007)和山西省科技攻关项目(20130311001-5)资助。 （31171839）
