作物学报Issue(2)：228-239,12.DOI:10.3724/SP.J.1006.2015.00228
枯萎病菌诱导感、抗陆地棉品种的转录因子表达变化
Expression Changes of Transcription Factors in Susceptible and Resistant Up-land Cotton (Gossypium hirsutum L.) Cultivars in Response to Fusarium wilt
摘要
Abstract
The Solexa sequencing technique was used to study the expression changes of transcription factors and their families in upland cotton cultivars susceptible and resistant to Fusarium wilt. A total of 39 transcription factor families and 433 transcription factors in Zhongmiansuo 12 and 52 transcription factor families and 588 transcription factors in Xinluzao 7 were changed in ex-pression in at least one comparison group induced by Fusarium wilt. The number of transcription factor families responsed to Fusarium wilt was more in Xinluzao 7 than in Zhongmiansuo 12, and the number of down-regulated genes was more than that of up-regulated genes in two varieties. After the induction by Fusarium wilt, in the process of time, the number of transcription factor families and transcription factors responsed to the Fusarium wilt in two cultivars showed the same changing trend of increase first and decrease then. The maximum number was reached after six hours in Zhongmiansuo 12, while at three hours after the inducing in Xinluzao 7. Among six comparison groups, there were nine overlapping transcription factors in six transcription factor families of Zhongmiansuo 12 and 31 overlapping transcription factors in 17 transcription factor families of Xinluzao 7. The expression of transcription factor showed strong cultivar specificity among varieties tolerant to Fusarium wilt. Except for 37 transcription factor families shared between two cultivars, two transcription factor families were unique to Zhongmiansuo 12 and Zhongmiansuo 15 transcription factor families were unique to Xinluzao 7.关键词
陆地棉/枯萎病/Solexa 测序/转录因子Key words
Upland cotton/Fusarium wilt/Solexa sequencing/Transcription factor引用本文复制引用
韩泽刚,赵曾强,何兰兰,柴蒙亮,李会会,张薇..枯萎病菌诱导感、抗陆地棉品种的转录因子表达变化[J].作物学报,2015,(2):228-239,12.基金项目
本研究由国家自然科学基金项目(31260358)和国家转基因生物新品种培育重大专项(2011ZX08005-005)资助。 （31260358）
