作物学报Issue(2)：308-317,10.DOI:10.3724/SP.J.1006.2015.00308
不同连作年限野生地黄根际土壤微生物群落多样性分析
Diversity Analysis of Rhizosphere Microflora of Wild R. glutinosa Grown in Monocropping for Different Years
摘要
Abstract
The soils sampled from the four different plots, including the newly planted, the two-year monocultured, the wild R. glutinosa and the control without growing R. glutinosa, were used to study the changes in microbial biomass and community composition using phospholipid fatty acid (PLFA) and terminal restriction fragment length polymorphism (T-RFLP) analyses. PLFA analysis indicated that the soil microbial community composition was significantly different among the R. glutinosa with different years of monoculture. Compared with the newly planted soil, the total PLFA content and the ratio of bacteria/fungi in two-year monocultured soil greatly declined. Further analysis by T-RFLP also displayed the distinct differences in rhizospheric bacterial community structure of R. glutinosa. The microbial compositions from the wild and the newly planted R. glutinosa soils tended to be more similar. It was found that the bacteria including Proteobacteria and Firmicutes were predominant in the wild and newly planted R. glutinosa soils. Some beneficial biocontrol bacteria (such as Bacillus, Pseudomonas, etc.) gathered in the rhizosphere of the wild and newly planted R. glutinosa. However, a large number of pathogenic bacteria bred in the rhizosphere of the two-year monocultured R. glutinosa, such as Clostridium sp., Flexibacter polymorphus, and Clostridium ghoni, and the num-ber of beneficial bacteria and cellulose degradation bacteria decreased. Furthermore, qRT-PCR analysis verified that the total number of Pseudomonas was much higher in the wild and newly planted R. glutinosa soils than in the two-year monocultured soil. In conclusion, the pathogenic microbes breed seriously in the rhizospheric soil of wild R. glutinosa under the monoculture regime, and yet the number of beneficial bacteria decline, resulting in weakened ability of wild R. glutinosa to resist the diseases so that the two-year monocultured wild R. glutinosa grows abnormally and its yield is decreased drastically.关键词
地黄/磷脂脂肪酸/末端限制性片段长度多态性/微生物多样性/植物根际Key words
Rehmannia glutinosa/PLFA/T-RFLP/Microbial diversity/Plant rhizosphere引用本文复制引用
吴林坤,黄伟民,王娟英,吴红淼,陈军,秦贤金,张重义,林文雄..不同连作年限野生地黄根际土壤微生物群落多样性分析[J].作物学报,2015,(2):308-317,10.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2012CB126309),国家自然基金联合基金项目(U1205021),国家自然科学基金项目(81303170)和福建农林大学优秀博士学位论文基金(324-1122yb005)资助。 （973计划）
