作物学报Issue(2)：339-346,8.DOI:10.3724/SP.J.1006.2015.00339
应用454测序技术分析种植制度对黑垆土微生物多样性的影响
Effect of Cropping Systems on Microbial Diversity in Black Loessial Soil Tested by 454 Sequencing Technology
摘要
Abstract
It has important significance on optimizing dryland cropping systems, mining the soil potential fertility and achieving the sustainable use of soil resources that we study the influence of cropping systems on soil microbial diversity in typical arid areas. In the long-term experiment lasted 27 years, we analyzed the diversity changes of bacteria and fungi in black loessial soil under different cropping systems in Loess Plateau using 454 sequencing technology. The results showed that bacterial diversity showed wheat-pea rotation>continuous wheat>fallow land>continuous alfalfa, and fungal diversity showed continuous wheat≈continuous alfalfa>fallow land>wheat-pea rotation under low nutritional stress with no fertilization. In the conditions of application of nitrogen fertilizer and phosphorus fertilizer, microbial diversity generally showed an decreasing and then increasing trend in long-period rotation of wheat–alfalfa (alfalfa→alfalfa→alfalfa→alfalfa→potato→wheat→wheat→wheat, eight years as a rotation period); Chao index and Shannon index of the 4th year alfalfa or the 1st year wheat were the lowest, and those of the 2nd year wheat were the maximum, with the bacteria Chao index and fungi Shannon index of 22.0% and 79.2% higher than those of continuous wheat, respectively. Microbial diversity generally showed an increasing trend in short-period rotation of wheat–sainfoin (sainfoin→wheat→wheat, three years as a rotation period), and that of the 2nd year wheat after sainfoin was the maximum, with fungi Chao index and Shannon index of 50.8% and 51.0% higher than that of continuous wheat respectively. Wheat-forage legumes rotation could improve the microbial diversity significantly in Loess dryland areas.关键词
454 测序/高通量测序/旱地土壤/微生物多样性/种植制度Key words
The 454 sequencing/High-throughput sequencing/Dry land soil/Microbial diversity/Cropping system引用本文复制引用
蔡艳,郝明德,张丽琼,臧逸飞,何晓雁..应用454测序技术分析种植制度对黑垆土微生物多样性的影响[J].作物学报,2015,(2):339-346,8.基金项目
本研究由国家科技支撑计划重大项目(2011BAD31B01)和宁夏农业综合开发科技推广项目(NTKJ-2013-03-1)资助。 （2011BAD31B01）
