作物学报Issue(3):367-377,11.
利用SRAP、ISSR、SSR标记绘制黄麻基因源分子指纹图谱
Construction of Molecular Fingerprinting Map in Gene Pool of Jute with SRAP, ISSR, and SSR Markers
摘要
Abstract
An experiment was conducted using 231 jute germplasm resources from abroad and at home to construct DNA finger-prints of jute varieties with SRAP, ISSR, SSR marker, and the DNA fingerprint analysis software. The results showed that 96 DNA fingerprints from 231 jute germplasm resources with 35 pairs of selected SRAP primers, 45 DNA fingerprints from 96 jute varieties with 11 selected polymorphic ISSR primers, and 13 DNA fingerprints from 48 jute varieties with 49 selected polymor-phic SSR primers were constructed. This study completed a total of 154 genomic DNA molecular fingerprint maps of jute varie-ties. Every identified jute variety had its unique “ID”. Other 77 local varieties had not been able to be identified due to their high genetic similarity with some varieties. It showed that jute local variety has a serious phenomenon of synonym.关键词
黄麻/遗传资源/分子标记/DNA指纹图谱Key words
Jute/Genetic resources/Molecular markers/DNA fingerprint引用本文复制引用
巫桂芬,祁建民,徐鲜均,徐建堂,陶爱芬,张立武,魏丽真,潘漠,方平平,林荔辉..利用SRAP、ISSR、SSR标记绘制黄麻基因源分子指纹图谱[J].作物学报,2015,(3):367-377,11.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-19-E06),引进国际先进农业科学技术计划(948计划)项目(2013-Z7)和国家科技支撑计划项目(2013BAD01B03-13)资助。 (CARS-19-E06)