作物学报Issue(3)：378-385,8.DOI:10.3724/SP.J.1006.2015.00378
棉花溶血磷酸酯酰转移酶(LPAT)家族基因的发掘和表达分析
Genome-wide Identification of Lysophosphatidic Acid Acyltransferase Gene Family and Their Expression Analysis in Cotton
摘要
Abstract
Metabolism related to lipids synthesis plays an important role in regulating both oil biosynthesis and fiber development in cotton. Lysophosphatidic acid acyltransferase (LPAT) is a key enzyme in oil biosynthesis pathway in plant. In this study, eight cotton LPAT family genes were identified and their gene sequences, chromosome location were obtained, based onG. raimondii genome database (http://www.phytozome.net/) and bioinformatic method. These LPAT members were anchored on six chromo-somes inG. raimondii. Phylogenetic analysis showed that LPAT candidate genes were classified into four groups, with two mem-bers each in group I and group III, one in group II, and three in group IV. The expression patterns of LPAT genes revealed their important roles in diverse functions in the developmental stages of vegetative and reproductive growth in cotton.LPAT6and LPAT8 showed the highest expression level in ovules at 17 days post-anthesis, which might play an important role in regulating oil biosynthesis. Eight genes showed the preferential expression level in fiber development stages. Among them,LPAT2,LPAT3, and LPAT4showed the higher expression level in fiber than in other tissues and organs, implying their association with cotton fiber development.关键词
油脂代谢/LPAT基因家族/染色体定位/系统分析/表达特征Key words
Lipids metabolism/LPAT gene family/Chromosome location/Phylogenetic analysis/Expression pattern引用本文复制引用
丁检,吴双,蔡彩平,郭旺珍..棉花溶血磷酸酯酰转移酶(LPAT)家族基因的发掘和表达分析[J].作物学报,2015,(3):378-385,8.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2011CB109300)和江苏高校优势学科建设工程资助项目(PAPD,010-809001)资助。 （973计划）
