作物学报Issue(3)：386-393,8.DOI:10.3724/SP.J.1006.2015.00386
云南3种野生稻中抗白叶枯病基因的鉴定
Identification of Bacterial Blight Resistance Gene in Yunnan Wild Rice
摘要
Abstract
Bacterial blight (BB) is one of the most destructive diseases in rice production. Developing resistance variety is the most efficient and economical solution to control this disease. Owing to the limited resistance genes from cultivated rice and the narrow spectrum of some BB resistance genes, wild rice become an important resource of BB resistance genes with wider spec-trum and higher resistance. In this study, seven cloned BB resistance genes were detected in 11 accessions (belonging toOryza rufipogon,O. officinalis, andO. granulata) of Yunnan wild rice by phenotypic and molecular marker identification. The results showed that Yunnan wild rice had different levels of resistance to BB strains Y8 and PXO99, particularly,O. granulatawas im-mune to Y8 and PXO99. The resistance genes xa5,xa13andXa21 were not found in all three wild rice materials. According to functional marker detection,O. rufipogon from Yuanjiang probably carryXa23 andXa3/Xa26;O. rufipogonfrom Jinghong probably carryXa1,Xa3/Xa26,andXa27; andO. officinalisandO. granulata probably carryXa3/Xa26 andXa27, respectively. These results provide a solid basis for exploring and cloning new BB resistance genes from Yunnan wild rice.关键词
野生稻/白叶枯病/抗性基因/PCR鉴定Key words
Wild rice/Bacterial blight/Resistance gene/PCR identification引用本文复制引用
李定琴,陈玲,李维蛟,柯学,余腾琼,李娥贤,黄兴奇,程在全..云南3种野生稻中抗白叶枯病基因的鉴定[J].作物学报,2015,(3):386-393,8.基金项目
本研究由国家自然科学基金项目(31000746,31160067, U1302265),国家公益性行业(农业)科研专项(201003021),云南省高端人才项目(2012HA002)和云南省应用基础研究项目(2010CD098)资助。 （31000746,31160067, U1302265）
