作物学报Issue(3)：414-421,8.DOI:10.3724/SP.J.1006.2015.00414
玉米小分子热激蛋白ZmHSP17.7基因的克隆与功能分析
Cloning and Function Analysis of Small Heat Shock Protein GeneZmHSP17.7 from Maize
摘要
Abstract
Heat shock proteins are typical proteins responding to high temperature. Maize germplasm POB21 was used to isolate a small heat-shock protein gene with a 477 bp CDS sequence. This gene, designatedZmHSP17.7, encodes a protein of 158 amino acids, which contains the typical ACD domain of HSP20 proteins. The predicted isoelectronic point and molecular weight of ZmHSP17.7 protein are 5.36 and 17.746 kD, respectively. The ZmHSP17.7 homologues were found in diverse plants, such as Arabidopsis and rice. According to the phylogenetic and subcellular localization analyses, ZmHSP17.7 is considered as a member of CI class of small heat shock protein family. The Northern blotting assay indicated that the expression ofZmHSP17.7was quickly induced by high temperature. Drought stress simulated by 15% PEG did not regulate the gene expression independently, but enhanced the heat-induced gene expression under combined stress of high temperature and drought. Exogenous ABA had no influence onZmHSP17.7 expression. Overexpression ofZmHSP17.7 inArabidopsis during seed germination and plant growth enhanced tolerance to high temperature and drought stresses. Our results indicate thatZmHSP17.7 may play a certain role in pro-tecting plant from high temperature, drought and their combination.关键词
玉米/小分子热激蛋白/高温/胁迫抗性Key words
Maize/Small heat shock protein/High temperature/Stress resistance引用本文复制引用
孙爱清,葛淑娟,董伟,单晓笛,董树亭,张杰道..玉米小分子热激蛋白ZmHSP17.7基因的克隆与功能分析[J].作物学报,2015,(3):414-421,8.基金项目
本研究由中国博士后科学基金(2012M511053)和国家转基因生物新品种培育重大专项(2013ZX08011-006)资助。 （2012M511053）
