作物学报Issue(3)：479-486,8.DOI:10.3724/SP.J.1006.2015.00479
飞机草类黄酮3’-羟化酶基因(CoF3’H)的克隆及其在烟草中的表达
Chromolaena odorata Flavonoid 3’-hydroxylase Gene Cloning and Its Expres-sion in Tobacco
摘要
Abstract
A flavonoid 3’-hydroxylase gene cDNA sequence was cloned by RACE and RT-PCR techniques fromChromolaena odorata. The obtained full-length cDNA was named asCoF3’H with GenBank accession number HQ268505.1. It is 1628 bp in length, containing a 1524 bp open reading frame, encoding 507 amino acid residues. The amino acid sequence ofCoF3’H con-tains cytochrome P450 domain and cysteine heme binding region (F××G×R×C×G). Homology analysis by DNAMAN software showed that the deducedCoF3’H protein was highly homologous to F3’H proteins from different species.CoF3’H gene was transferred into tobacco byAgrobacterium-mediated genetic transformation. There was higher content of flavonvoids in T2 trans-genic tobacco than in the wild type. This result shows thatCoF3’H plays an important role in flavonoid biosynthesis, and provides a basis for researches on allelopathy and comprehensive utilization ofC. odorata in the future.关键词
飞机草/类黄酮3’-羟化酶/克隆/表达Key words
Chromolaena odorata/Flavonoid 3’-hydroxylase/Clone/Expression引用本文复制引用
何海旺,潘华清,张铙丹,何龙飞..飞机草类黄酮3’-羟化酶基因(CoF3’H)的克隆及其在烟草中的表达[J].作物学报,2015,(3):479-486,8.基金项目
本研究由广西自然科学基金项目(桂科自0832047)资助。 （桂科自0832047）
