作物学报Issue(3)：499-506,8.DOI:10.3724/SP.J.1006.2015.00499
甘蔗CIPK基因的同源克隆与表达
Cloning and Expression Analysis ofCIPK Gene in Sugarcane
摘要
Abstract
CIPK (calcineurin B-like-interacting protein kinase) is a plant specific class of serine / threonine protein kinases, which plays an important role in plant response to stress, especially relates with the signal transduction for biotic stresses (drought, high salt, ABA). According to the primers designed on the conserved domain ofCIPK15gene fromZea mays, a full-length cDNA se-quence of serine/threonine kinase gene termed asScCIPK was cloned by RT-PCR method from sugarcane (Saccharum Complex). The sequence analysis showed thatScCIPK had a length of 1782 bp containing the open reading frame (ORF, 91–1631 bp), which encoded 513 amino acids residues with two conserved domains (Kc-like superfamily and AMPKA-C-like superfamily). The characters predicted based on the bioinformatics analysis revealed that theScCIPK gene of sugarcane was a soluble acidic protein, which has two conserved functional domains with the main function for central_intermediary_metabolism, and its protein was located in endoplasmic reticulum (membrane). The mainly secondary structure element wasα-helix. Real-time quantitative PCR(RT-qPCR) analysis revealed that the expression ofScCIPKwas higher in bud than in other tissues, meanwhile the inducible expression level ofScCIPK was most significantly up-regulated under the ABA stress, 5.3 times higher than that of control, which suggested thatScCIPK most probably involves in sugarcane resistance to drought and osmotic stresses. The results in this study could provide a basis of cloning and functional identification of other members ofScCIPKin sugarcane and promote the use of ScCIPK gene in sugarcane genetic engineering.关键词
甘蔗/CIPK基因/同源克隆/生物信息学/实时定量PCRKey words
Sugarcane/CIPK/homology cloning/Bioinformatics/Real-time PCR引用本文复制引用
黄珑,苏炜华,张玉叶,黄宁,凌辉,肖新换,阙友雄,陈如凯..甘蔗CIPK基因的同源克隆与表达[J].作物学报,2015,(3):499-506,8.基金项目
本研究由国家自然科学基金项目(31340060),福建省高等学校新世纪优秀人才支持计划项目(JA14095)和国家现代农业产业技术体系建设专项(CARS-20)资助。 （31340060）
