作物学报Issue(5)：673-682,10.DOI:10.3724/SP.J.1006.2015.00673
小麦脂质转运蛋白基因TaLTP克隆及功能分析
Cloning and Functional Analysis of Lipid Transfer Protein Gene TaLTP in Wheat
摘要
Abstract
Lipid transfer protein (LTP) is a kind of small molecular protein, which name stands for its ability to transfer lipid be-tween cell membranes in plant. LTP plays a key role in cuticle synthesis and adaptation to abiotic stress. We cloned a full-length cDNA sequence of TaLTP gene encoding a lipid transfer protein from wheat (Triticum aestivum L.), which length is 510 bp with a 339 bp open reading frame. The cDNA sequence contains 112 amino acids with a N-terminal signal peptide within the first 25 amino acids. This gene contains eight cysteine residues conserved in amino acid sequences. TaLTP-s was detected to be located between markers WMC449 and WMC93 on chromosome 1A in wheat, with genetic distances of 2.1 cM and 5.9 cM, respectively. The result of subcellular localization exhibited that TaLTP-s is located in the cell membrane and cytoplasm. TaLTP-s was ex-pressed in all tissues at flowering stage, including leaf, root, floret, anther and pistil, and mature seeds of wheat. The highest ex-pression level was identified in the floret, especially in the anther. TaLTP-s was up-regulated by ABA, PEG, NaCl, and 4ºC treat-ments which indicates that TaLTP-s is involved in different signal pathways responding to abiotic stress. Arabidopsis thaliana overexpressing TaLTP-s showed higher cell membrane stability and survival rate than the controls under salinity stress. These results provide a candidate gene for wheat improvement in response to salt and other abiotic stresses.关键词
小麦/脂质转运蛋白/基因克隆/非生物胁迫/耐盐性Key words
Wheat/TaLTP-s/Gene cloning/Abiotic stress/Salt tolerance引用本文复制引用
李倩,王景一,毛新国,李昂,高丽锋,刘惠民,景蕊莲..小麦脂质转运蛋白基因TaLTP克隆及功能分析[J].作物学报,2015,(5):673-682,10.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2011AA100501,2012AA10A308)资助。 （863计划）
