作物学报Issue(5):692-697,6.DOI:10.3724/SP.J.1006.2015.00692
水稻抗白叶枯病新基因Xa39分子标记有效性的评价
Evaluation of Selective Efficiency of Molecular Markers Linked with a Novel Gene Xa39 Resistant to Bacterial Blight in Rice
摘要
Abstract
Xa39 is a novel and dominant resistance gene with broad spectrum for bacterial leaf blight in rice. In previous study, this gene was mapped on chromosome 11. In present study, FF329 carrying Xa39 gene as donor parent and BT4, BT6, BT12, BT18 as recipients were used to develop four F2 segregation populations (4FL10, 4FL14, 4FL17, 4FL21). Combining artificial inoculation with phenotype identification of bacterial leaf blight, the validity of three molecular markers tightly linked with Xa39, and two co-dominant molecular markers was investigated for marker-assisted selection (MAS). The results showed that the accu-racy of marker RM26985 for MAS was 95.81%and that of marker RM206 was 93.61%in the four populations. The accuracy of two markers RM26985 and RM206 used simultaneously for MAS was up to 95.35%. Therefore, RM26985 and RM206 can be used as effective markers in MAS for Xa39 in rice breeding program.关键词
Xa39/水稻白叶枯病/SSR/MASKey words
Xa39/Rice bacterial blight/SSR/MAS引用本文复制引用
卓大龙,胡丹丹,张帆,石英尧,高用明,周永力,黎志康..水稻抗白叶枯病新基因Xa39分子标记有效性的评价[J].作物学报,2015,(5):692-697,6.基金项目
本研究由国家自然科学基金项目(U1201211)和国家高技术研究发展计划(863计划)项目(2014AA10A603)资助。 (U1201211)