作物学报Issue(5)：806-812,7.DOI:10.3724/SP.J.1006.2015.00806
31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测
Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 31 Wild Core Sugarcane Germplasms
摘要
Abstract
The objectives of this study was to clarify the level of resistance to Puccinia melanocephala and the distribution of Bru1 in sugarcane wild germplasm collections. Resistance of 31 wild core germplasm from National Nursery of Sugarcane Germ-plasm Resources (NNSGR, Kaiyuan, China) was identified at seedling stage, and the resistance gene Bru1 was detected in 2013. The results showed that 28 out of 31 materials (90.3%) were highly resistant (Grade 1) or moderately resistant (Grade 3). Of them, nineteen (61.3%) were highly resistant (Grade 1), three (9.7%) were resistant (Grade 2) and six (19.4%) were moderately resistant. Bru1 was detected in eight (25.8%) resistant materials (Guizhou 78-2-12, Yunnan 97-4, E. rockii 95-19, E. rockii 95-20, Yunnan 83-224, Guangxi 79-8, Yunnan 95-35, and Guangxi 89-13). The absence of Bru1 in other 20 resistant and three susceptible entries suggests they may contain brown rust resistance-associated genes other than Bru1. Therefore the elite brown rust resistance gene should be contained in numerous sugarcane wild core germplasm collections of NNSGR, and be valuable for future brown rust resistant breeding programs in sugarcane.关键词
甘蔗/野生核心种质/抗褐锈病基因Bru1/分子检测Key words
Sugarcane/Wild core germplasm/Brown rust resistance gene Bru1/Molecular detection引用本文复制引用
李文凤,王晓燕,黄应昆,张荣跃,单红丽,尹炯,罗志明..31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测[J].作物学报,2015,(5):806-812,7.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-20-2-2),云南省现代农业产业技术体系建设专项和云南省科技计划项目(2013BB013)资助。 （CARS-20-2-2）
