作物学报Issue(6)：889-899,11.DOI:10.3724/SP.J.1006.2015.00889
大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应
Epistatic and QTL × Environment Interaction Effects of QTLs for Leaf Traits and Leaf Chlorophyll Content in Soybean
摘要
Abstract
An RIL population containing 447 lines, derived from a cross of cultivar Jingdou 23 × Huibuzhiheidou, as well as their parents were used to analyze inheritance and detect epistatic effects, and QTL × environment (QE) interactions related to leaf traits and leaf chlorophyll content (CC) in soybean using major gene plus polygene mixed inheritance analysis and composite interval mapping (QTL NETwork 2.0). The leaf traits including leaf length (LL), leaf width (LW), leaf stalk length (LSL) were evaluated in 2011, 2012, and 2013, as well as CC was detected on Aug. 1 and Aug. 8, 2012, and on Aug. 2 and Aug. 9, 2013. LL was found to be controlled by two pairs of additive-additive by additive epistatic hybrid main genes, LW was found to be con-trolled by three pairs of equivalent main genes, LSL was found to be controlled by four pairs of additive-additive by additive epistatic major genes, CC was controlled by four pairs of additive major genes. Ten QTLs for LL, LW, LSL, and CC were mapped on the linkage group (LG) A1, A2, C2, H_1, L, and O, respectively. Of them two QTLs for LL were mapped on LG C2 and LG L, additive by additive epistatic effect and QE interactions. Three QTLs with additive effect and QE interactions associated with LW were mapped on LG A2, C2, and O. Two QTLs for LSL were mapped on LG L and O. Three QTLs for CC were mapped on LG A1, C2, and H_1. The genetic mechanism for leaf traits and leaf chlorophyll content is more complicated containing additive ef-fect, additive × additive epistatic effect and QE interaction. It is important to consider not only to QTLs with major effects, but also to those with epistatic effects in soybean molecular marker-assisted breeding for stability of expression and inheritance of agronomic traits.关键词
大豆/叶片性状/叶绿素含量/QTL与环境互作效应/上位互作效应Key words
Soybean/Leaf traits/Leaf chlorophyll content/QTL × environment interactions effects/Epistatic effects引用本文复制引用
梁慧珍,余永亮,杨红旗,董薇,许兰杰,牛永光,张海洋,刘学义,方宣钧..大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应[J].作物学报,2015,(6):889-899,11.基金项目
本研究由河南省科技创新杰出人才计划项目(114200510002),国家转基因生物新品种培育重大专项(2009ZX08018-001B,2011ZX08004-005,2014ZX0800402B),河南省重点科技攻关计划项目(132102110091)和河南省农业科学院专项(201315603,201315615,201218334)资助。 （114200510002）
