作物学报Issue(8):1172-1182,11.DOI:10.3724/SP.J.1006.2015.01172
河南小麦新育成品种(系)白粉病抗性鉴定与分子标记检测
Identification and Molecular Detection of Powdery Mildew Resistance of New Bred Wheat Varieties (Lines) in Henan Province, China
摘要
Abstract
The powdery mildew resistance of 809 (2009–2013) and 412 (2009–2012) new bred wheat varieties (lines) from Henan provincial regional trials was tested usingBlumeria graminis f. sp.tritici(Bgt) isolates E09 and E20, respectively. Molecular markers linked to powdery mildew resistance genesPm2,Pm4a,Pm8, andPm21were used to detect the presence of the individ-ual resistance gene. Among the 908 varieties (lines) inoculated by E09, 199 (21.9%) exhibited resistance. Among the 412 varieties (lines) inoculated by E20, 39 (9.5%) exhibited resistance. Only 15 (3.6%) varieties (lines) showed resistance to both E09 and E20. The 1RS chromatin was detected in 580 out of 908 (63.9%) varieties (lines), indicating the common use ofPm8 or newly devel-oped 1BL/1RS translocation lines in wheat breeding program. Two varieties carried the board spectrum powdery mildew resis-tance genePm21 originating from 6AL/6VS translocation. Eight and two lines might contain thePm2andPm4aloci, respectively. Six varieties (lines) seemed to carry at least two powdery mildew resistance genes. Our results indicated that the newly developed wheat varieties (lines) in Henan province are important powdery mildew resistance resources. However, the common use of 1BL/1RS translocations and a few powdery mildew resistance genes has revealed a very narrow genetic diversity of the resistant resource. It is very urgent to introduce new diversified and broad spectrum powdery mildew resistance genes into the commercial wheat breeding program.关键词
小麦品种/抗白粉病基因/分子标记/抗性鉴定Key words
Wheat varietiey/Resistance gene to powdery mildew/Molecular markers/Resistance identification引用本文复制引用
曹廷杰,陈永兴,李丹,张艳,王西成,赵虹,刘志勇..河南小麦新育成品种(系)白粉病抗性鉴定与分子标记检测[J].作物学报,2015,(8):1172-1182,11.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2011CB100104)资助。 (973计划)