作物学报Issue(8)：1183-1190,8.DOI:10.3724/SP.J.1006.2015.01183
普通小麦DH155抗白粉病基因的分子作图及应用分子标记辅助选择将其转移
Molecular Mapping of Powdery Mildew Resistance GeneMlDH155in Hexap-loid Wheat DH155 and Its Transfer by Marker Assisted Selection
摘要
Abstract
Hexaploid wheat (Triticum aestivum L.) line DH155 is highly resistant to wheat powdery mildew caused byBlumeria graminisf. sp.tritici(Bgt). To identify theBgtresistance gene(s) in DH155, we developed an F2 population and its derived F2:3 families by crossing the resistant line DH155 with the susceptible line SN2890. The segregation ratios indicated that the seedling resistance toBgtE09 in DH155 was controlled by a single dominant gene, which was tentatively designatedMlDH155. By bulked segregation analysis, two codominant SSR markers,Xcfd81andXcfd18, were identified to be linked toMlDH155. To identify the closely linked markers to the targeted gene, we developed five new molecular markers based on the published D genome se-quences of Chinese Spring andAegilops tauschii, which permitted mapping ofMlDH155 within an interval of 1.0 cM, flanked by XsdauK525 andXsdauK527. ThePm resistant line DH155 was crossed with two elite wheat lines (HB133-4 and Han 10) but sus-ceptible to powdery mildew. Subsequently, two powdery mildew resistant lines with the genetic background of HB133-4 and one resistant line with Han 10 background were developed by genotypic and phenotypic selection, which were designated by the name of SDAU2100, SDAU2101 and SDAU2102, respectively. Among the 14Bgtisolates tested at the seedling stage, DH155 was resistant to 13 and susceptible to 1 isolates. The virulence pattern of theseBgtisolates on DH155 was similar to that of the known powdery mildew resistance genePm2, but the reactions of DH155 to twoBgtisolates differed from those of Ulka/8*Cc carrying Pm2. Compared to previous studies aboutPm2, MlDH155was most likely to be either the same as or an allele ofPm2.关键词
小麦/DH155/白粉病/抗病基因/分子标记Key words
Triticum aestivum/DH155/Powdery mildew/Resistance gene/Molecular marker引用本文复制引用
管昌英,郭军,薛凤博,张广旭,王宏伟,李安飞,孔令让..普通小麦DH155抗白粉病基因的分子作图及应用分子标记辅助选择将其转移[J].作物学报,2015,(8):1183-1190,8.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2012AA101105)和引进国际先进农业科学技术计划(948计划)国际合作项目(2013-S19)资助。 （863计划）
