中国现代医生Issue(14)：69-72,75,5.
柯萨奇病毒A组16型VP1蛋白的原核表达
Prokaryotic expression of coxsackievirus group A type 16 VP1 protein
王志宏 1丁莹莹 2冯娇娇 2李连青 3潘卫 2郭存九1
作者信息
- 1. 山西医科大学第二附属医院检验科，山西太原 030001
- 2. 第二军医大学病原生物学教研室，上海 200433
- 3. 山西省临床检验中心，山西太原 030001
- 折叠
摘要
Abstract
Objective To construct the recombinant plasmid of coxsackievirus group A type 16 (CA16)VP1,express in E.coli and identify expressed product. Methods The CA16 VP1 gene was amplified by PCR,The constructed recombi-nant plasmid pET21b-CA16 VP1 was transformed into E.coli BL21(DE3) for expession and purification,expressed and purified product was identified by SDS-PAGE,Indirect ELISA tested Antigenicity and validity of recombinant proteins CA16 VP1. Results The coxsackievirus group A type 16 VP1 protein was expressed in peokaryotic cells and purified, which showed specific reaction with mice antiserum against CA16 virus,high reactivity with part serums of the elderly population in Taiyuan city. Conclusion CA16 VP1 protein was successfully expressed, had good Antigenicity and va-lidity,which will lay a foundation for study on the CA16 diagnostic kits.关键词
柯萨奇病毒A组16型/VP1蛋白/原核表达/酶联免疫吸附Key words
Coxsackievirus group A type 16(CA16)/VP1 protein/Peokaryotic expression/Enzyme-linked immunosor-bent assay分类
医药卫生引用本文复制引用
王志宏,丁莹莹,冯娇娇,李连青,潘卫,郭存九..柯萨奇病毒A组16型VP1蛋白的原核表达[J].中国现代医生,2014,(14):69-72,75,5.
