中国现代医生Issue(35)：1-4,4.
吡格列酮对急性脑缺血再灌注损伤大鼠TNF-α、IL-10和细胞凋亡的影响
Influence of pioglitazone on tissue TNF-α,IL-10 and cell apoptosis with cerebral ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate the changes of TNF-α, IL-10 and cell apoptosis of cerebral ischemia-reperfusion injury and the influence of Pioglitazone(PGZ) on these parameters in rats. Methods Eighty-four male SD rats were ran-domly divided into two groups, PGZ group (n=42) and the control group (n=42). The PGZ group was treated with improved Zea-longa method and received tail vein injections of PGZ (10 mg/kg) immediately after injury, the control group re-ceived tail vein injections with the same dose sodium chloride injection immediately, after injury and repeat one time everyday until the rats was killed. Each group was divided into seven subgroups by sacrificed time after injury, those were 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, and 7 d, each subgroup got 6 rats. Each subgroup were randomly selected three rats after being killed, detected the expression of TNF-α and IL-10 of rats contusion peri tissues brain tissue by using im munohistochemical methods, while using TUNEL method to observe the peri cell apoptosis after brain contusion. Results The expression of TNF-α in each PGZ group was significantly decreased but the IL-10 was significant ly increased compared with the control group (P<0.05),and a significant negative correlation between the both of parameters in two groups as well (P<0.01);At the same time the number of apoptotic cells was decreasing (P<0.05). Conclusion Pioglita-zone is probably through the route of relieving inflammation response, reducing the change of secondary brain injury af-ter traumatic brain injury and decreasing neural cell apoptosis, and then provide protection of neurocytes.关键词
吡格列酮/脑缺血再灌注损伤/TNF-α/IL-10/细胞凋亡Key words
Pioglitazone/Cerebral ischemia reperfusion/TNF-α/IL-10/Cell apoptosis分类
医药卫生引用本文复制引用
赵俞,余智,卢波达,刘凯的,方来明..吡格列酮对急性脑缺血再灌注损伤大鼠TNF-α、IL-10和细胞凋亡的影响[J].中国现代医生,2014,(35):1-4,4.基金项目
广西自然科学基金 （）
