中外医学研究Issue(14)：144-145,2.
人ERCC1基因启动子荧光素酶报告基因质粒的构建
Construction of the Human ERCC1 Luciferase Reporter Plasmid Promoter
宋莹 1谷依学 1仇秦威 1贺智敏1
作者信息
- 1. 广州医科大学附属肿瘤医院 广东 广州 510095
- 折叠
摘要
Abstract
Objective:To construct a human excision repair cross complementation 1(ERCC1) promoter luciferase reporter plasmid.Method:The ERCC1 promoter from human genomic DNA was amplified by PCR,and was inserted into the pGL4 Basic vector.The amplified DNA sequence was confirmed by sequencing.Result:DNA sequencing verified the successful construction of the plasmid pGL4-ERCC1-P1.Conclusion:The human ERCC1 promoter luciferase reporter gene vector is successfully constructed,which can be use for further study on gene expression regulation.关键词
报告基因/ERCC1/启动子Key words
ERCC1/Luciferase reporter gene/Promoter引用本文复制引用
宋莹,谷依学,仇秦威,贺智敏..人ERCC1基因启动子荧光素酶报告基因质粒的构建[J].中外医学研究,2014,(14):144-145,2.
