植物营养与肥料学报Issue(2)：372-379,8.DOI:10.11674/zwyf.2014.0213
施用不同生物有机肥对连作黄瓜枯萎病防治效果及其机理初探
Effects and mechanisms of application with different bio-organic fertilizers in controlling Fusarium wilt of cucumber
摘要
Abstract
Two kinds of strains , Bacillus subtilis SQR9 and Trichoderma harzianum T37 which are antagonistic to Fusarium oxysporum, were used in this study .Bioorganic fertilizers containing the antagonists either in nursery or in transplanted soils were applied to investigate the effects of three types of bio-organic fertilizers ( BIOⅠ, BIOⅡand BIOⅢ) on the controlling efficiency of Fusarium wilt in cucumber growth , and profile the colonization of these two strains on cucumber roots .Results showed:1 ) As control , the incidence rate in pot experiment was assigned 100%, and the treatment with organic fertilizer ( OF) was 88.2%, while the incidence rates of treated with BIOⅠ, BIOⅡBIOⅢ were 51.0%, 19.6% and 13.7%, respectively .2 ) The biomasses of cucumber treated with BIOⅠ, BIOⅡand BIOⅢ, were 2.55 , 2.46 and 2.58 times of the control , respectively .3 ) Application of BIOⅡand BIOⅢ could reduce the quantity of Fusarium oxysporum in rhizosphere of cucumber to ×103 copies/g soil detected by real-time PCR, but ×107 copies/g soil of Fusarium oxysporum was found in the control .SQR9 and T37 could be effectively colonized on the cucumber root surface and thus occupied the ecological sites in the rhizosphere .Study suggested that application of BIO could not only effectively suppress the occurrence of Fusarium wilt in cucumber , but also promote the cucumber growth .关键词
连作障碍/枯草芽孢杆菌/哈茨木霉/黄瓜枯萎病/生物有机肥/定殖Key words
continuous mono-cropping obstacle/Bacillus subtilis/Trichoderma harzianum/Fusarium oxysporum f. sp.cucumerinum/bio-organic fertilizer/colonization分类
农业科技引用本文复制引用
袁玉娟,胡江,凌宁,仇美华,沈其荣,杨兴明..施用不同生物有机肥对连作黄瓜枯萎病防治效果及其机理初探[J].植物营养与肥料学报,2014,(2):372-379,8.基金项目
农业部农业公益性行业专项（201103004）；农业部948滚动项目（2011-G27）资助。 （）
