植物营养与肥料学报Issue(2)：445-458,14.DOI:10.11674/zwyf.2014.0222
外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase 及矿质营养吸收的影响
Effects of exogenous NO on mineral nutrition absorption, lipid peroxidation and ATPase of plasma membrane in Catharanthus roseus tissues under cadmium stress
摘要
Abstract
Heavy metal pollution has become one of the major problems in the world , and soil cadmium ( Cd ) pollution is the most widespread pollution .Cd is a heavy metal without biological functions and highly toxic to organism , and its accumulation in soil is becoming a severe threat to plant growth .Nitric oxide ( NO ) is an oxidation reduction signaling molecule and a reactive nitrogen species ( RNS) with diverse physiological functions in plants.Recently, many researchers found that NO plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plant kingdom .The valuable ornamental plant Catharanthus roseus is an important landscaping and anticancer drug source plant which is widely distributed in city gardens and on roadsides in China.Our objective was to investigate the effects of exogenous NO with the sodium nitroprusside ( SNP, an exogenous NO donor ) on the plant growth , reactive oxygen species ( O-2 and H2 O2 ) , antioxidative enzymes , ATPase enzyme and mineral nutrition absorption in C.roseus seedlings under cadmium stress .A controlled pot-experiment was arranged with seven concentrations ( CK, 0;Cd, 25 mg/kg Cd2+;T1, Cd2++0.45 mg/kg SNP;T2, Cd2++0.90 mg/kg SNP; T3, Cd2++1.80 mg/kg SNP; T4, Cd2++3.60 mg/kg SNP; T5, Cd2++7.20 mg/kg SNP).Morphological, ecological, physiological indexes were observed during the growth of C.roseus.The distribution of Cd , macro ( K, Ca and Mg ) and micronutrients ( Fe, Cu and Zn ) in C.roseus plants was determined after harvest .The results show that the addition of 25 mg/kg CdCl2 inhibits the growth of C.roseus seedlings, and dramatically increases accumulation of Cd in both shoots and roots , furthermore, the absorptions of macro and micronutrients are inhibited .Addition of 0.45 mg/kg, 0.90 mg/kg and 1.80 mg/kg SNP significantly decrease the transport of Cd from roots to shoots , alleviate the inhibition of K, Ca, Mg and Fe, Cu, Zn absorption induced by cadmium , reduce the toxicity symptoms , and promote the plant growth .The accumulation of ROS , including malondialdehyde ( MDA) and superoxide radicals ( O-2 ) generation rate , significantly is increased in C. roseus seedlings exposed to the cadmium stress , and is resulted in the lipid peroxidation , which is indicated by accumulated concentration of thiobarbituric acid-reactive substances ( TBARS).Addition of 0.45 mg/kg, 0.90 mg/kg and 1.80 mg/kg SNP significantly decrease the levels of O-2 and H2 O2 , and lipid peroxidation .The activities of antioxidant enzymes also show the same changes .Addition of 0.45 , 0.90 and 1.80 mg/kg SNP increase the activities of catalase ( CAT ) , superoxide dismutase ( SOD ) , peroxidase ( POD ) , and glutathione ( GSH) in C.roseus seedlings exposed to Cd .Simultaneously , addition of 0.45 mg/kg, 0.90 mg/kg and 1.80 mg/kg SNP can induce H +-ATPase, Ca2+-ATPase and 5′-AMPase enzyme activity of plasma membrane to normal levels in shoots and roots .Addition of 1.80 mg/kg exogenous SNP has the most significant alleviating effect against cadmium toxicity , while the addition of 3.60 mg/kg and 7.20 mg/kg SNP have no significant effects with cadmium treatments.关键词
长春花/镉胁迫/外源 NO/质膜氧化/ATP 酶/矿质营养Key words
Catharanthus roseus/cadmium stress/exogenous NO/lipid peroxidation/ATPase/mineral nutrition分类
农业科技引用本文复制引用
刘柿良,潘远智,杨容孑,丁继军,何杨,王力,马明东..外源一氧化氮对镉胁迫下长春花质膜过氧化、ATPase 及矿质营养吸收的影响[J].植物营养与肥料学报,2014,(2):445-458,14.基金项目
四川农业大学“211工程”双支计划项目资助。 （）
