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RP-HPLC法测定六味千骨巴布剂中柚皮苷的含量

刘世萍 郭美华 李小红 马妍妍 白建海 王琪

中医药学报Issue(2):49-51,3.
中医药学报Issue(2):49-51,3.

RP-HPLC法测定六味千骨巴布剂中柚皮苷的含量

Determination of Naringin in Liuwei Qiangu Cataplasm by RP-HPLC

刘世萍 1郭美华 1李小红 1马妍妍 1白建海 2王琪1

作者信息

  • 1. 哈尔滨医科大学附属第一医院药学部,黑龙江哈尔滨150001
  • 2. 哈尔滨医科大学附属第一医院眼科,黑龙江哈尔滨150001
  • 折叠

摘要

Abstract

Objective: To establish a method for the determination of Naringin in Liuwei Qiangu Cataplasm by RP -HPLC.Methods:The HyperClone 5u BDS C18 130A column (250mm ×4.6mm,5micron) was used,and the mobile phase was methand -waters-acetic (45:55:2).The flow rate was 1.0mL/min;the column temperature was 28℃;the detection wavelength was 284 nm.Results: The linear range of Naringin was within 0.061 6μg ~1.232μg ( r =0.999 9).The average recovery was 98.34%,RSD=1.04%(n=6).Conclusion:The method is simple,fast,and the result is accurate and reliable .It can be used for quality control of the Naringin in Liuwei Qiangu Cataplasm .

关键词

六味千骨巴布剂/反相高效液相色谱法/柚皮苷

Key words

Liuwei Qiangu Cataplasm/RP-HPLC/Naringin

分类

医药卫生

引用本文复制引用

刘世萍,郭美华,李小红,马妍妍,白建海,王琪..RP-HPLC法测定六味千骨巴布剂中柚皮苷的含量[J].中医药学报,2014,(2):49-51,3.

基金项目

黑龙江省中医药科研项目 ()

中医药学报

OACSTPCD

1002-2392

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