中医药学报Issue(3)：18-22,5.
寒凝证类痛经大鼠各时段子宫微循环的变化及不同针刺刺激量的干预效应
Effect of Different Quantity of A cupuncture Stimulus on Uterine Microcirculation in Dysmenorrhea Rats withCold Syndrome at Different Time-points
摘要
Abstract
Objective:To observe the effect of different quantity of acupuncture stimulus on uterine microcirculation in dysmenorrhea rats with cold syndrome at different time -points,and to discuss the relationship between quantity of acu-puncture stimulus and acupuncture effect .Methods:Dioestrum female rats were randomly divided into four groups , sa-line control group, model control group, A stimuli group(“deqi”group), B stimuli group(no“deqi”group), with eight rats in each group .Rats of model control group and stimulus groups were frozen in -25℃freezer for five days with four hours per day while given subcutaneous estradiol benzoate injection for ten days with one hour after the last injection , in-tra-peritoneal injection of oxytocin 2u/each.Saline control group was given the same dose of saline for ten days with one hour after the last injection , intra-peritoneal injection of saline 2u/each.On the tenth day, stimulus groups were given different quantity of acupuncture stimulus on both sides of “Sanyinjiao(SP6)” for 20 minutes.Use XW-B-3 Cold Light microcirculation tester to observe the changes of uterine microcirculation in each group at different time -points.Results:Model control group was compared with to saline control group , and diameters of micro -vessel at all time-points, diameters of capillary (except 40min and 60min) were significantly contracted (P<0.01),blood flow slo-wer or stagnation (P<0.01 or P<0.05).Stimulus groups were compared with model control group , and about A stim-uli group, diameters of micro-vessel at all time -points, blood flow at all time -points was speeded up significantly (P<0.01).About B stimuli group, diameters of micro -vessel were expanded only at 30min-point(P<0.01).A stimuli group was compared with B stimuli group :diameters of micro -vessel at all time -points, diameters of capillary (only 5min,10min,20min and 30min)were significantly expanded (P<0.01 or P<0.05),blood flow(except 50min and 60min)were obviously improved(P<0.01 or P<0.05).Conclusion:Acupuncture could improve uterine microcir-culation.With different quantity of acupuncture stimulus , effects of uterine microcirculation were different between A stimuli group and B stimuli group ,the former was in a better and stronger“deqi”state,which showed the importance of“deqi”in acupuncture efficacy .关键词
寒凝痛经/子宫微循环/刺激量Key words
Dysmenorrhea with cold syndrome/Uterine microcirculation/Quantity of acupuncture stimulus分类
医药卫生引用本文复制引用
沈小雨,李晓泓,朱江,任晓暄,张露芬,郭孟玮,杨佳敏,赵雅芳,许咏思,罗丽,齐丹丹,张玲,申松希..寒凝证类痛经大鼠各时段子宫微循环的变化及不同针刺刺激量的干预效应[J].中医药学报,2014,(3):18-22,5.基金项目
国家重点基础研究发展计划（973计划）课题（No．2012CB518506，No．2006CB504503）；国家自然科学基金面上项目（No．30973793）；教育部博士点基金项目 （）
