中医药学报Issue(4)：53-57,5.
蒙药槟榔十三味丸（高尤-13）对抑郁模型大鼠行为学及不同脑区单胺类神经递质的影响
Effects of Mongolian Pharmaceutical Betel Shisanwei Ingredient Pills on the Behavior and Different Cerebral Region Monoamine Neurotransmitters in the Rat Models with Depressive Chronic Stress
摘要
Abstract
Objective:To study the effects on the behaviors and prefrontal cortex and hippocampus monoamine neurotrans -mitters and their metabolites in the chronic depressed rat models to probe the anti -depression mechanism of Mongolian pharmaceutical Betel Shisanwei ingredient pills .Methods:Sixty male Wistar rats were randomly divided into six groups according to the sugar consumption test and body weight (10 rats in each group):Normal control group,Model group, Fluoxetine group and High dose,medium dose and low dose groups of Betel Shisanwei ingredient pills .Except the normal control group,other groups were treated with the chronic unpredictable mild stress stimulation combined with lonely rai -sing for 21days.10mL/kg drugs were given to the rats,once daily,continuously for 21 days.After 21 days,behavior chan-ges of rats were detected through Open -field test,and sugar consumption test and body weight were also measured . HPLC -ECD techniques were used to analyze the levels of prefrontal cortex and hippocampus NE and 5 -HT and DA and their metabolites.Results:Compared with normal control group ,the scores of horizontal activity and vertical activity , sugar consumption decreased significantly in the model group (P <0.01 or P <0.05);Compared with model group,Flu-oxetine group and Betel Shisanwei ingredient pills group got significantly higher scores of horizontal activity ,vertical ac-tivity and sugar consumption (P <0.05 or P <0.01).HPLC -ECD techniques showed that the model rats prefrontal cortex NE,5 -HT,DA,DOPAC,hippocampus 5 -HT,5HIAA,DA content decreased more significantly than those in normal control group(P <0.05 or P <0.01).Betel Shisanwei ingredient pills could increase model rat 's prefrontal cortex NE,5 -HT,DA,DOPAC and hippocampus 5 -HT,5HIAA,DA content, especially the medium and high dose groups were significant(P <0.05 or P <0.01).Conclusion: Betel Shisanwei ingredient pills can improve abnormal behavior of depressive model rats, improve the neurotransmitter NE, 5 -HT, DA and its metabolic product content in rats brain, and it could be one of the important antidepressant effect mechanisms of Betel Shisanwei ingredient pills .关键词
槟榔十三味丸/抑郁症/行为学/皮质/海马/神经递质Key words
Betel Shisanwei ingredient pills/Depression/Behavioral science/Cortex/Hippocampus/Neurotransmitter分类
医药卫生引用本文复制引用
佟海英,王旭,乌吉斯古冷,白亮凤,包伍叶,胡日乐巴根,李婧,张月..蒙药槟榔十三味丸（高尤-13）对抑郁模型大鼠行为学及不同脑区单胺类神经递质的影响[J].中医药学报,2014,(4):53-57,5.基金项目
北京市自然科学基金项目 （）
