中医药学报Issue(6)：22-24,3.
GC－MS同时测定石菖蒲挥发油中β－细辛醚、α－细辛醚和甲基丁香酚的含量
Simultaneous Determin taion ofβ-asarone,α-asarone and Methyl Eugenol in Volatile Oil from Acorus Tatarinowii Schottby GC-MS
王知斌 1李晶 1雷印廷 1王秋红 1杨炳友 1匡海学1
作者信息
- 1. 黑龙江中医药大学教育部北药基础与应用研究重点实验室，黑龙江省中药天然药物药效物质基础研究重点实验室，黑龙江哈尔滨150040
- 折叠
摘要
Abstract
Objective:To develop a GC-MS method for determination ofβ-asarone,α-asarone and methyl eugenol in Acorus Tatarinowii Schott volatile oil.Methods:A GC-MS-SIM method was established.The column was DB-1701 capillary column (60 m ×0.25 mm ×0.25μm) .The sample volume was 1μL with a split ratio of 5:1 .The carrier gas was 99.99%high purity Helium.Ionization was performed in electron impact ionization (EI) mode at 70 eV.Selected ions were m/z 208 forβ-asarone,α-asarone and m/z 178 for methyl eugenol.Results:The linear range was 525~5250 ng/ml forβ-asarone, 60~600 ng/ml forα-asarone and 50~500 ng/ml for methyl eugenol.The average recov-eries ofβ-asarone,α-asarone and methyl eugenol were 105.5%, 103.6%and 101.8% respectively.Conclusion:This method is accurate and sensitive, and it can apply to simultaneously determine the concentrations of β-asarone,α-asarone and methyl eugenol in Acorus Tatarinowii Schott volatile oil .关键词
β-细辛醚/α-细辛醚/甲基丁香酚/气质联用Key words
β-Asarone/α-Asarone/Methyl eugenol/GC-MS分类
医药卫生引用本文复制引用
王知斌,李晶,雷印廷,王秋红,杨炳友,匡海学..GC－MS同时测定石菖蒲挥发油中β－细辛醚、α－细辛醚和甲基丁香酚的含量[J].中医药学报,2014,(6):22-24,3.
