中医药学报Issue(1)：50-53,4.
HPLC法同时测定牛黄解毒片中黄芩苷、黄芩素、芦荟大黄素、大黄素的含量
HPLC Method for Simultaneous Determination of Baicalin, Baicalein,Aloe Emodin and Emodin in Niuhuang Jiedu Tablets
摘要
Abstract
Objective:To establish a method for the determination of multi-components ( baicalin, baicalein, aloe emod-in and emodin) in Niuhuang Jiedu tablets ( NHJDT) and perfect the quality control standards.Methods:Using Agilent ZORBAX SB-C18(4.6 ×250mm,5μm) as the separation column, the UV absorbance was monitored at 260 nm by in-jecting 10μL of the sample solution each time and the column temperature was maintained at30 ℃.The flow rate was 1.0mL/min.Acetonitrile was as the mobile phase A;0.1%phosphoric acid was as mobile phase B for gradient elution. Results:The linear range in content determination of baicalin, baicalein, aloe emodin and emodin was followed by 5.06~202.4μg/mL,1.836~30.6μg/mL,0.196~7.84μg/mL and 0.992~19.84μg/mL,the correlation coefficient R2 was in turn 0.999 7,0.999 1,0.999 8 and 0.999 8.The average recoveries were 99.17%(RSD=0.72%), 97.46%(RSD=0.85%), 97.91%(RSD=1.97%), 98.23%(RSD=0.82%)(n=6).Conclusion:The method is accurate and can be used to evaluate the quality of NHJDT.关键词
HPLC/牛黄解毒片/含量测定Key words
HPLC/Niuhuang Jiedu tablets/Content determination分类
医药卫生引用本文复制引用
潘丽丽,李亭亭,杨颂,郝变,袁少雄,许晓嘉,徐新房,李向日..HPLC法同时测定牛黄解毒片中黄芩苷、黄芩素、芦荟大黄素、大黄素的含量[J].中医药学报,2015,(1):50-53,4.基金项目
北京中医药大学研究生自主课题（2014－JYBZZ －XS －081）；中国药典2015版“中成药多组分控制方法的应用研究”；保健食品中违禁物质检测技术研究 （）
