中医药学报Issue(2)：27-30,4.
一株半夏内生青霉属真菌次级代谢产物及生物活性的研究
The Secondary Metabolites Isolated from One Endophytic Fungi of Pinellia Ternate and Biological Activity
摘要
Abstract
Objective:To isolate the secondary metabolites from the fermentation broth and mycelium of an endophytic fun-gi BX-9 ( Penicillium sp.) isolated from Pinellia ternate ( Thunb.) and detect the biological activity of these secondary metabolites.Methods:The strain BX-9 was fermented in PDB culture,and fermentation broth was extracted by ethyl acetate,and the mycelia of endophytic were extracted in methanol for two times.The chemical constituents of the strain BX-9 were isolated by variety of chromatographyic methods.The AChE inhibitory and antitumor activity was deter-mined by modified Ellman’ s method and MTT method.Results:Seven compounds were isolated from the fermentation broth and mycelium of endophytic fungi from Pinellia ternate and they were identified as 2-Hydroxy-14-tricosenvic acid (1), [4, 6, 8, 22-tetraen-3-one-ergostane] (2),Ergosterol (3),Ergosterol peroxide (4),( +) -Brefel-din A (5), 7-oxobrefeldin A (6), 4-epi-Brefeldin A (7).All of the compounds had no AChE inhibitory activity. Compounds (3~6) had a remarkably antitumor activity against Hela,7721, HL60.Conclusion:It is possible to isolate antitumor compounds from endophytic fungi of Pinellia ternate and the strain BX-9 can produce large numbers of brefel-din A.关键词
半夏/内生真菌/青霉属/抗肿瘤/乙酰胆碱酯酶抑制活性Key words
Pinellia ternate/Endophytic fungi/Penicillium/Antitumor/Inhibitory activity of acetylcholinesterase分类
医药卫生引用本文复制引用
杨中铎,魏博,薛鹏辉..一株半夏内生青霉属真菌次级代谢产物及生物活性的研究[J].中医药学报,2015,(2):27-30,4.基金项目
国家自然科学基金项目（21262022）；浙江省自然科学基金项目 （）
