中医药学报Issue(2)：30-34,5.
中药天花粉对人恶性黑色素瘤细胞形态、增殖、周期和迁移的影响
Effect of Radix Trichosanthis Decoction on Cell Morphology, Cell Proliferation and Cell Migration in Human Melanoma A375 Cells
摘要
Abstract
Objective:To investigate the effect of Radix Trichosanthis decoction on cell morphology, cell proliferation and cell migration in human melanoma A375 cells in vitro.Methods:Use the microscope and MTT method and the changes of the cellular morphology and cell proliferation were detected in A375 cells treated by Radix Trichosanthis decoction;flo wing cytometry was applied to detect the cell cycle in A375 cells;cell migration was evaluated by using scratch experi-ment and the effect of the cell migration was assessed by the scarification test.Results: Compared with the control group, we found that A375 cells treated by Radix Trichosanthis decoction had the lowest cell viability and the number of normal morphology cells was significantly reduced in A375 cells in concentration-dependent manner.A375 cells trea-ted with Radix Trichosanthis decoction showed that cell proliferation was more obviously suppressed than those untreated control using MTT assay.Flowing cytometry assay showed the percentage of G2 phase cells had a significant difference compared with the control group treated by different concentration Radix Trichosanthis decoction.The cell scratch analy-sis indicated A375 cells treated by 640 and 2 560μg/mL Radix Trichosanthis decoction had lower wound healing rate. Conclusion:We found Radix Trichosanthis decoction could cause cell death and cell morphology abnormal in vitro.We also found Radix Trichosanthis decoction could inhibit cell proliferation and cell migration in concentration-dependent manner.Interestingly, G2 phase arrest could be induced by Radix Trichosanthis decoction.Our findings can provide new knowledge about Radix Trichosanthis decoction in development of melanoma and indicate the potential application of Radix Trichosanthis decoction in the prognosis of melanoma.关键词
天花粉/恶性黑色素瘤/细胞增殖周期/细胞迁移Key words
Radix Trichosanthis/Malignant melanoma/Cell proliferation cycle/Cell migration分类
医药卫生引用本文复制引用
杨颖,刘巧,胡俊媛,王俭,杨恺瑞,胡秀华..中药天花粉对人恶性黑色素瘤细胞形态、增殖、周期和迁移的影响[J].中医药学报,2015,(2):30-34,5.基金项目
国家自然科学基金项目（ No．81274044）；国家级大学生创新创业训练计划课题 （）
