中医药学报Issue(3)：13-16,4.
护肝片中5种木脂素成分的高效液相－荧光检测研究
Simultaneous Determination of Five Lignin of Hugan Tablets by HPLC and Fluorescence Detection
摘要
Abstract
Objective:To establish a simultaneous determination of five markers, namely schisandrol A and B, schisan-drin A-C for evaluation and quality control of Hugan tablets by high performance liquid chromatography (HPLC) com-bined with fluorescence detection ( FLD) .Methods:The simultaneous HPLC-FLD analysis of Hugan tablets was recor-ded at excitation wavelength of 254nm and emission wavelength of 330nm by using a reversed phase C18 column (4.6mm ×250mm, 5 μm) by an isocratic elution mobile phase consisting of methanol and water (85:15) within 25min at 35℃with a flow rate of 0.6mL/min.Results: In the quantitative analysis, all calibration curves of five selected markers showed good linear regression ( R2≥0.999 0) within the tested ranges, and the recovery of the method was in the range of 84.21%~105.04%, with RSD values ranging from 1.17%to 2.18%.Conclusion:The results demonstrate that the developed method is a rapid and effective analytical method for the quality assessment of Hugan tablets.关键词
护肝片/HPLC/荧光检测器/联苯环辛二烯型木脂素Key words
Hugan tablets/HPLC/Fluorescence Detection/Dibenzo ( a,c) cyclooctene Lignans分类
医药卫生引用本文复制引用
孙黎明,梁军,王杰姝,郭信东,夏永刚,匡海学..护肝片中5种木脂素成分的高效液相－荧光检测研究[J].中医药学报,2015,(3):13-16,4.基金项目
国家科技部国家重大新药创制专项（2012 ZX09304005004）；黑龙江省博士后启动基金（ LBH－Q13158）；黑龙江省博士后特别资助项目 （）
