中医药信息Issue(2)：70-72,3.
HPLC-ELSD测定复方血栓通胶囊中三七皂苷R1、人参皂苷Rg1及Rb1的含量
Simultaneous Assay of Notoginsenoside R1,Ginsenoside Rg1 and Rb1 in Fufang Xueshuantong Capsules by HPLC-ELSD
摘要
Abstract
Objective:To establish simultaneous assay of the contents of notoginsenoside R 1 , ginsenoside Rg 1 and Rb1 in Fufang Xueshuantong capsules by HPLC -ELSD.Methods: The column was packed with a Dia-monsilC18 (4.6mm ×250mm, 5μm) stationary phase.The mobile phase consisted of acetonitrile (A) -water (B), eluted in gradient mode(0 min→5min→20min,A:20%→25%→45%) with flow rate of 1.0mL/min. Column temperature was set at 25℃.The temperature of driftube was 70℃ and the air flow rate was 2.0L/min.Results:The linear ranges of notoginsenoside R1, ginsenoside Rg1and Rb1 were 0.3~1.5μg (r=0. 9994), 1.5~7.5μg (r=0.9995 )and 1.5~7.5μg(r=0.9999) respectively;the average recoveries (n=6) were 98.8%,98.2% and 97.6%,with the RSD of 1.1%,1.8% and 1.3% respectively.Conclusion:The method is rapid , simple and accurate .It can be used for quality evaluation of Fufang Xueshuantong cap-sules.关键词
HPLC-ELSD/三七皂苷R1/人参皂苷Rg1/人参皂苷Rb1/复方血栓通胶囊Key words
HPLC -ELSD/Notoginsenoside R1/Ginsenoside Rg1/Ginsenoside Rb1/Fufang Xueshuantong capsules分类
医药卫生引用本文复制引用
王玉华,付丽佳,徐莲琴,杨宝峰..HPLC-ELSD测定复方血栓通胶囊中三七皂苷R1、人参皂苷Rg1及Rb1的含量[J].中医药信息,2014,(2):70-72,3.基金项目
王玉华（1974-），女，副主任药师，主要从事医院药学工作。 （）
