中医药信息Issue(4)：16-19,4.
二氢槲皮素对 H2 O2诱导 H9 c2心肌细胞损伤的保护作用
Protective Effects of Dihydroquercetin against H2 O2 -induced Injury in H9c2 Cardiomyoblasts
摘要
Abstract
Objective:To investigate the protective effects of dihydroquercetin against H 2 O2 -induced injury in H9c2 cardiomyoblasts.Methods:A model of H2O2 -induced injury in H9c2 cardiomyoblasts was established. Cell viability was determined colorimetrically by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazo-lium (MTT) assay.The supernates and cells were collected , respectively, after the different treatments for measuring the LDH and MDA levels, as well as SOD,CAT, and GSH-Px activity with the corresponding de-tection kit according to the manufacturer ’ s instructions .Results:H9 c2 cells were pretreated with dihydroquer-cetin for 12 h before exposure to 150 μM for 6 h.H2 O2 treatment caused severe injury to H9c2 cardiomyo-blasts.Dihydroquercetin pretreatment could attenuate H 2 O2 -induced injury , decrease the levels of LDH leakage and MDA, and increase the activities of SOD, CAT, and GSH-Px with a dose-dependent.Conclu-sion:Dihydroquercetin showed significantly protective effects against H 2 O2 -induced injury in H9c2 cardio-myoblasts in dose-dependent .The mechanism of this may be due to its increasing the activities of endogenous antioxidant enzymes , as well as improving the stabilization of myocardial cells .关键词
二氢槲皮素/H2 O2/H9c2心肌细胞/抗氧化/活性氧自由基Key words
Dihydroquercetin/H2 O2/H9c2 cardiomyoblasts/Anti-oxidation/ROS分类
医药卫生引用本文复制引用
王知斌,翟亚东,苏晓琳,吕金南,于莹,王秋红,杨炳友,匡海学..二氢槲皮素对 H2 O2诱导 H9 c2心肌细胞损伤的保护作用[J].中医药信息,2014,(4):16-19,4.基金项目
黑龙江中医药大学杰出人才培育基金（No．2012jc02）；高等学校博士学科点专项科研基金（No．20132327120002）；黑龙江中医药大学“优秀创新人才支持计划”科研项目 （）
