中医药信息Issue(4)：20-22,3.
贝母花的定性鉴别及含量测定研究
Study on TLC and Content Determination for Flowe rs of Fritillaria Thunbergii Miq
摘要
Abstract
To establish the quality standard for flowers of Fritillaria thunbergii Miq.Methods:Flowers of Fritillaria thunbergii Miq.were identified by TLC .The contents of peimine and peiminninne were determined by ELSD-HPLC:The optimal conditions of separation and detection were achieved on a C18 ana-lytical column with an mobile phase consisting of acetonitrile -water -triethylamine ( 68∶32∶0 .03 ) at the flow-rate of 1.0 mL/min;the temperature for the detector drift tube was set at 90℃and the nitrogen flow-rate was at 2.2L/min, 20μL as the injection volume .Results:The identification by TLC was highly specific . The calibration curves for peimine and peiminine were linear over the range of 0 .0185~0 .185 mg/mL (r=0.9998 ) and 0.0188~0.188 mg/mL(r=0.9997) , respectively .The average recovery rate was 100.40%with RSD of 1.50%(n=6).Conclusion:The method is simple and reproducible . It can serve as a quantita-tive determination and quality control of flowers of Fritillaria thunbergii Miq.关键词
浙贝母/贝母素甲/贝母素乙/薄层鉴别/含量测定Key words
Fritillaria thunbergii Miq ./Peimine/Peiminninne/Qualitative identification/Content determina-tion分类
医药卫生引用本文复制引用
崔明超,程斌,陈宏降,陈云..贝母花的定性鉴别及含量测定研究[J].中医药信息,2014,(4):20-22,3.基金项目
浙江医药高等专科学校校级科研课题资助项目（ No． ZPCSR2010007）；浙江省教育厅2013年高等学校访问工程师校企合作项目 （）
