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甘草地上部分总黄酮含量测定方法的筛选

程新宇 韩亚男 康雪芳 侯俊玲 王文全

中医药信息Issue(2):1-5,5.
中医药信息Issue(2):1-5,5.

甘草地上部分总黄酮含量测定方法的筛选

Selection of Content Determination Methods of Total Flavonoids of the Aerial Part of Licorice

程新宇 1韩亚男 1康雪芳 1侯俊玲 1王文全2

作者信息

  • 1. 北京中医药大学中药学院,北京 100102
  • 2. 中国医学科学院北京协和医学院药用植物研究所,北京 100193
  • 折叠

摘要

Abstract

Objective:To examine the impact of different flavonoid references and different color reactions as an indicator to the determination of total flavonoids of the aerial part of licorice.Methods:Choose Liquiritin,ru-tin, quercetin as references, AlCl3 colorimetric method , 10% KOH chromogenic method, NaNO2 -Al (NO3)3chromogenic method, determine the content of total flavonoids of the aerial parts of licorice by UV spectrophotometry.Results:Determination of total flavonoid contents by different references and color reac-tions, the results were quite different.Conclusion : When we determine the content of total flavonoids of the aerial part of licorice by ultraviolet spectrophotometry, quercetin can be used as reference, 10%KOH as chro-mogenic agent chromogenic method.

关键词

甘草/总黄酮/甘草苷/槲皮素/芦丁/含量测定/分光光度法

Key words

Licorice/Total flavonoids/Liquiritin/Quercetin/Tutin/Content determination/UV Spectrophotome-try

分类

医药卫生

引用本文复制引用

程新宇,韩亚男,康雪芳,侯俊玲,王文全..甘草地上部分总黄酮含量测定方法的筛选[J].中医药信息,2015,(2):1-5,5.

基金项目

国家“重大新药创制”科技重大专项项目( No.2009ZX09308-002);国家中医药管理局行业专项项目 ()

中医药信息

OACSTPCD

1002-2406

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