中医药信息Issue(2):1-5,5.
甘草地上部分总黄酮含量测定方法的筛选
Selection of Content Determination Methods of Total Flavonoids of the Aerial Part of Licorice
摘要
Abstract
Objective:To examine the impact of different flavonoid references and different color reactions as an indicator to the determination of total flavonoids of the aerial part of licorice.Methods:Choose Liquiritin,ru-tin, quercetin as references, AlCl3 colorimetric method , 10% KOH chromogenic method, NaNO2 -Al (NO3)3chromogenic method, determine the content of total flavonoids of the aerial parts of licorice by UV spectrophotometry.Results:Determination of total flavonoid contents by different references and color reac-tions, the results were quite different.Conclusion : When we determine the content of total flavonoids of the aerial part of licorice by ultraviolet spectrophotometry, quercetin can be used as reference, 10%KOH as chro-mogenic agent chromogenic method.关键词
甘草/总黄酮/甘草苷/槲皮素/芦丁/含量测定/分光光度法Key words
Licorice/Total flavonoids/Liquiritin/Quercetin/Tutin/Content determination/UV Spectrophotome-try分类
医药卫生引用本文复制引用
程新宇,韩亚男,康雪芳,侯俊玲,王文全..甘草地上部分总黄酮含量测定方法的筛选[J].中医药信息,2015,(2):1-5,5.基金项目
国家“重大新药创制”科技重大专项项目( No.2009ZX09308-002);国家中医药管理局行业专项项目 ()